The enzyme biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation by EC 188.8.131.52, biotin carboxylase. The carboxyl group is eventually transferred to acetyl-CoA by EC 184.108.40.206, acetyl-CoA carboxytransferase. In some organisms the carrier protein is part of EC 220.127.116.11, acetyl-CoA carboxylase.
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