Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+). The enzyme binds the closed form of the sugar and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism . The enzyme catalyses the aldose-ketose isomerization of several sugars. Most enzymes also catalyse the reaction of EC 188.8.131.52, L-fucose isomerase . The enzyme from the bacterium Falsibacillus pallidus also converts D-altrose to D-psicose . cf. EC 184.108.40.206, L-arabinose isomerase.
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Takeda, K., Yoshida, H., Izumori, K. and Kamitori, S. X-ray structures of Bacillus pallidus D-arabinose isomerase and its complex with L-fucitol. Biochim. Biophys. Acta1804 (2010) 1359–1368. [PMID: 20123133]