| EC |
5.3.1.4 |
| Accepted name: |
L-arabinose isomerase |
| Reaction: |
β-L-arabinopyranose = L-ribulose |
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For diagram of L-Arabinose catabolism, click here |
| Other name(s): |
L-arabinose ketol-isomerase; araA (gene name) |
| Systematic name: |
β-L-arabinopyranose aldose-ketose-isomerase |
| Comments: |
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) [2]. The enzyme binds β-L-arabinopyranose [4] and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism [6]. The enzyme can also convert α-D-galactose to D-tagatose with lower efficiency [5]. |
| Links to other databases: |
BRENDA, EXPASY, GTD, KEGG, MetaCyc, PDB, CAS registry number: 9023-80-7 |
| References: |
| 1. |
Heath, E.C., Horecker, B.L., Smyrniotis, P.Z. and Takagi, Y. Pentose formation by Lactobacillus plantarum. II. L-Arabinose isomerase. J. Biol. Chem. 231 (1958) 1031–1037. [PMID: 13539034] |
| 2. |
Patrick, J.W. and Lee, N. Purification and properties of an L-arabinose isomerase from Escherichia coli. J. Biol. Chem. 243 (1968) 4312–4318. [PMID: 4878429] |
| 3. |
Nakamatu, T. and Yamanaka, K. Crystallization and properties of L-arabinose isomerase from Lactobacillus gayonii. Biochim. Biophys. Acta 178 (1969) 156–165. [DOI] [PMID: 5773448] |
| 4. |
Schray, K.J. and Rose, I.A. Anomeric specificity and mechanism of two pentose isomerases. Biochemistry 10 (1971) 1058–1062. [DOI] [PMID: 5550812] |
| 5. |
Cheetham, P.S.J. and Wootton, A.N. Bioconversion of D-galactose into D-tagatose. Enzyme and Microbial Technology 15 (1993) 105–108. |
| 6. |
Banerjee, S., Anderson, F. and Farber, G.K. The evolution of sugar isomerases. Protein Eng. 8 (1995) 1189–1195. [DOI] [PMID: 8869631] |
| 7. |
Manjasetty, B.A. and Chance, M.R. Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production. J. Mol. Biol. 360 (2006) 297–309. [DOI] [PMID: 16756997] |
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| [EC 5.3.1.4 created 1961, modified 2022] |
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