The Enzyme Database

Your query returned 11 entries.    printer_iconPrintable version

Accepted name: lysophospholipase
Reaction: 2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate
Other name(s): lecithinase B; lysolecithinase; phospholipase B; lysophosphatidase; lecitholipase; phosphatidase B; lysophosphatidylcholine hydrolase; lysophospholipase A1; lysophopholipase L2; lysophospholipase transacylase; neuropathy target esterase; NTE; NTE-LysoPLA; NTE-lysophospholipase
Systematic name: 2-lysophosphatidylcholine acylhydrolase
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9001-85-8
1.  Abe, M., Ohno, K. and Sato, R. Possible identity of lysolecithin acyl-hydrolase with lysolecithin-lysolecithin acyl-transferase in rat-lung soluble fraction. Biochim. Biophys. Acta 369 (1974) 361–370.
2.  Contardi, A. and Ercoli, A. The enzymic cleavage of lecithin and lysolecithin. Biochem. Z. 261 (1933) 275–302.
3.  Dawson, R.M.C. Studies on the hydrolysis of lecithin by Penicillium notatum phospholipase B preparation. Biochem. J. 70 (1958) 559–570. [PMID: 13607409]
4.  Fairbairn, D. The preparation and properties of a lysophospholipase from Penicillium notatum. J. Biol. Chem. 173 (1948) 705–714. [PMID: 18910725]
5.  Shapiro, B. Purification and properties of a lysolecithinase from pancreas. Biochem. J. 53 (1953) 663–666. [PMID: 13032127]
6.  van den Bosch, H., Aarsman, A.J., De Jong, J.G.N. and van Deenen, L.L.M. Studies on lysophospholipases. I. Purification and some properties of a lysophospholipase from beef pancreas. Biochim. Biophys. Acta 296 (1973) 94–104. [DOI] [PMID: 4693514]
7.  van den Bosch, H., Vianen, G.M. and van Heusden, G.P.H. Lysophospholipase-transacylase from rat lung. Methods Enzymol. 71 (1981) 513–521. [PMID: 7278668]
8.  van Tienhoven, M., Atkins, J., Li, Y. and Glynn, P. Human neuropathy target esterase catalyzes hydrolysis of membrane lipids. J. Biol. Chem. 277 (2002) 20942–20948. [DOI] [PMID: 11927584]
9.  Quistad, G.B., Barlow, C., Winrow, C.J., Sparks, S.E. and Casida, J.E. Evidence that mouse brain neuropathy target esterase is a lysophospholipase. Proc. Natl. Acad. Sci. USA 100 (2003) 7983–7987. [DOI] [PMID: 12805562]
10.  Lush, M.J., Li, Y., Read, D.J., Willis, A.C. and Glynn, P. Neuropathy target esterase and a homologous Drosophila neurodegeneration-associated mutant protein contain a novel domain conserved from bacteria to man. Biochem. J. 332 (1998) 1–4. [PMID: 9576844]
11.  Winrow, C.J., Hemming, M.L., Allen, D.M., Quistad, G.B., Casida, J.E. and Barlow, C. Loss of neuropathy target esterase in mice links organophosphate exposure to hyperactivity. Nat. Genet. 33 (2003) 477–485. [DOI] [PMID: 12640454]
[EC created 1961, modified 1976, modified 1983]
Accepted name: wax-ester hydrolase
Reaction: a wax ester + H2O = a long-chain alcohol + a long-chain carboxylate
Other name(s): jojoba wax esterase; WEH
Systematic name: wax-ester acylhydrolase
Comments: Also acts on long-chain acylglycerol, but not diacyl- or triacylglycerols.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 66625-78-3
1.  Huang, A.H.C., Moreau, R.A. and Liu, K.D.F. Development and properties of a wax ester hydrolase in the cotyledons of jojoba seedlings. Plant Physiol. 61 (1978) 339–341. [PMID: 16660288]
2.  Moreau, R.A. and Huang, A.H.C. Enzymes of wax ester catabolism in jojoba. Methods Enzymol. 71 (1981) 804–813.
[EC created 1984]
Accepted name: phorbol-diester hydrolase
Reaction: phorbol 12,13-dibutanoate + H2O = phorbol 13-butanoate + butanoate
For diagram of reaction, click here
Other name(s): diacylphorbate 12-hydrolase; diacylphorbate 12-hydrolase; phorbol-12,13-diester 12-ester hydrolase; PDEH
Systematic name: 12,13-diacylphorbate 12-acylhydrolase
Comments: Hydrolyses the 12-ester bond in a variety of 12,13-diacylphorbols (phorbol is a diterpenoid); this reaction inactivates the tumour promotor 12-O-tetradecanoylphorbol-13-acetate from croton oil.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 81181-74-0
1.  Shoyab, M., Warren, T.C. and Todaro, G.J. Isolation and characterization of an ester hydrolase active on phorbol diesters from murine liver. J. Biol. Chem. 256 (1981) 12529–12534. [PMID: 6946062]
[EC created 1984]
Accepted name: phosphatidylinositol deacylase
Reaction: 1-phosphatidyl-D-myo-inositol + H2O = 1-acylglycerophosphoinositol + a carboxylate
Other name(s): phosphatidylinositol phospholipase A2; phospholipase A2
Systematic name: 1-phosphatidyl-D-myo-inositol 2-acylhydrolase
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 81604-94-6
1.  Gray, N.C.C. and Strickland, K.P. The purification and characterization of a phospholipase A2 activity from the 106,000 x g pellet (microsomal fraction) of bovine brain acting on phosphatidylinositol. Can. J. Biochem. 60 (1982) 108–117. [PMID: 7083039]
2.  Gray, N.C.C. and Strickland, K.P. On the specificity of a phospholipase A2 purified from the 106,000 X g pellet of bovine brain. Lipids 17 (1982) 91–96. [DOI] [PMID: 7087686]
[EC created 1984]
Accepted name: sialate O-acetylesterase
Reaction: N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate
Other name(s): N-acetylneuraminate acetyltransferase; sialate 9(4)-O-acetylesterase; sialidase
Systematic name: N-acyl-O-acetylneuraminate O-acetylhydrolase
Comments: Acts on free and glycosidically bound N-acetyl- or N-glycoloyl-neuraminic acid; acts mainly on the 4-O- and 9-O-acetyl groups. Also acts on some other O-acetyl esters, both cyclic and acyclic compounds, which are not sialic acids.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 89400-31-7
1.  Garcia-Sastre, A., Villar, E., Manuguerra, J.C., Hannoun, C. and Cabezas, J.A. Activity of influenza C virus O-acetylesterase with O-acetyl-containing compounds. Biochem. J. 273 (1991) 435–441. [PMID: 1991039]
2.  Shukla, A.K. and Schauer, R. High performance liquid chromatography of enzymes of sialic acid metabolism. Hoppe-Seyler's Z. Physiol. Chem. 363 (1982) 1039–1040.
[EC created 1984]
Accepted name: acetoxybutynylbithiophene deacetylase
Reaction: 5-(4-acetoxybut-1-ynyl)-2,2′-bithiophene + H2O = 5-(4-hydroxybut-1-ynyl)-2,2′-bithiophene + acetate
Other name(s): acetoxybutynylbithiophene esterase; 5-(4-acetoxy-1-butynyl)-2,2′-bithiophene:acetate esterase
Systematic name: 5-(4-acetoxybut-1-ynyl)-2,2′-bithiophene O-acetylhydrolase
Comments: The enzyme is highly specific.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 82346-63-2
1.  Sütfeld, R. and Towers, G.H.N. 5-(4-Acetoxy-1-butinyl)-2,2′-bithiophene:acetate esterase from Tagetes patula. Phytochemistry 21 (1982) 277–279.
[EC created 1986]
Accepted name: acetylsalicylate deacetylase
Reaction: acetylsalicylate + H2O = salicylate + acetate
Other name(s): aspirin esterase; aspirin esterase; acetylsalicylic acid esterase; aspirin hydrolase
Systematic name: acetylsalicylate O-acetylhydrolase
Comments: Not identical with EC (carboxylesterase), EC (arylesterase), EC (acetylcholinesterase) or EC (cholinesterase). The activity of the liver cytosol enzyme is highest with acetyl esters of aryl alcohols, and thioesters are also hydrolysed; the microsomal enzyme also hydrolyses some other negatively charged esters, with highest activity on esters of salicylate with long-chain alcohols.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 87348-04-7
1.  Ali, B. and Kaur, S. Mammalian tissue acetylsalicylic acid esterase(s): identification, distribution and discrimination from other esterases. J. Pharmacol. Exp. Ther. 226 (1983) 589–594. [PMID: 6875867]
2.  Kim, D.-H., Yang, Y.-S. and Jakoby, W.B. Aspirin hydrolyzing esterases from rat liver cytosol. Biochem. Pharmacol. 40 (1990) 481–487. [DOI] [PMID: 2383281]
3.  White, K.N. and Hope, D.B. Partial purification and characterization of a microsomal carboxylesterase specific for salicylate esters from guinea-pig liver. Biochim. Biophys. Acta 785 (1984) 138–147. [DOI] [PMID: 6704404]
[EC created 1986, modified 1989]
Accepted name: methylumbelliferyl-acetate deacetylase
Reaction: 4-methylumbelliferyl acetate + H2O = 4-methylumbelliferone + acetate
Other name(s): esterase D
Systematic name: 4-methylumbelliferyl-acetate acylhydrolase
Comments: Acts on short-chain acyl esters of 4-methylumbelliferone, but not on naphthyl, indoxyl or thiocholine esters.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 83380-83-0
1.  Hopkinson, D.A., Mestriner, M.A., Cortner, J. and Harris, H. Esterase D: a new human polymorphism. Ann. Hum. Genet. 37 (1973) 119–137. [DOI] [PMID: 4768551]
[EC created 1986]
Accepted name: 2-pyrone-4,6-dicarboxylate lactonase
Reaction: 2-oxo-2H-pyran-4,6-dicarboxylate + H2O = (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
For diagram of the protocatechuate 3,4-cleavage pathway, click here
Other name(s): 2-pyrone-4,6-dicarboxylate hydrolase; 2-pyrone-4,6-dicarboxylate lactonohydrolase
Systematic name: 2-oxo-2H-pyran-4,6-dicarboxylate lactonohydrolase
Comments: The product is most likely the keto-form of 4-oxalomesaconate (as shown in the reaction) [1,2]. It can be converted to the enol-form, 4-hydroxybuta-1,3-diene-1,2,4-trioate, either spontaneously or by EC, 4-oxalomesaconate tautomerase [3].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, UM-BBD, CAS registry number: 84177-55-9
1.  Kersten, P.J., Dagley, S., Whittaker, J.W., Arciero, D.M. and Lipscomb, J.D. 2-Pyrone-4,6-dicarboxylic acid, a catabolite of gallic acids in Pseudomonas species. J. Bacteriol. 152 (1982) 1154–1162. [PMID: 7142106]
2.  Maruyama, K. Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase. J. Biochem. (Tokyo) 93 (1983) 557–565. [PMID: 6841353]
3.  Nogales, J., Canales, A., Jiménez-Barbero, J., Serra B., Pingarrón, J. M., García, J. L. and Díaz, E. Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida. Mol. Microbiol. 79 (2011) 359–374. [DOI] [PMID: 21219457]
[EC created 1986, modified 2010]
Accepted name: N-acetylgalactosaminoglycan deacetylase
Reaction: N-acetyl-D-galactosaminoglycan + H2O = D-galactosaminoglycan + acetate
Other name(s): polysaccharide deacetylase (misleading); Vi-polysaccharide deacetylase; N-acetyl galactosaminoglycan deacetylase
Systematic name: N-acetyl-D-galactosaminoglycan acetylhydrolase
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 52410-59-0
1.  Jorge, J.A., Kinney, S.G. and Reissig, J.L. Purification and characterization of Neurospora crassa N-acetyl galactosaminoglycan deacetylase. Braz. J. Med. Biol. Res. 15 (1982) 29–34. [PMID: 6217857]
[EC created 1986]
Accepted name: juvenile-hormone esterase
Reaction: (1) juvenile hormone I + H2O = juvenile hormone I acid + methanol
(2) juvenile hormone III + H2O = juvenile hormone III acid + methanol
For diagram of juvenile hormone biosynthesis, click here
Glossary: juvenile hormone I = methyl (2E,6E,10R,11S)-10,11-epoxy-7-ethyl-3,11-dimethyl-2,6-tridecadienoate
juvenile hormone I acid = (2E,6E,10R,11S)-10,11-epoxy-7-ethyl-3,11-dimethyl-2,6-tridecadienoate
juvenile hormone III = methyl (2E,6E,10R)-10,11-epoxy-3,7,11-trimethyldodeca-2,6-dienoate
juvenile hormone III acid = (2E,6E,10R)-10,11-epoxy-3,7,11-trimethyldodeca-2,6-dienoate
Other name(s): JH-esterase; juvenile hormone analog esterase; juvenile hormone carboxyesterase; methyl-(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate acylhydrolase
Systematic name: methyl-(2E,6E,10R)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate acylhydrolase
Comments: Demethylates the insect juvenile hormones JH1 and JH3, but does not hydrolyse the analogous ethyl or isopropyl esters.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 50812-15-2
1.  de Kort, C.A.D. and Granger, N.A. Regulation of the juvenile hormone titer. Annu. Rev. Entomol. 26 (1981) 1–28.
2.  Mitsui, T., Riddiford, L.M. and Bellamy, G. Metabolism of juvenile hormone by the epidermis of the tobacco hornworm (Manduca sexta). Insect Biochem. 9 (1979) 637–643.
[EC created 1989, modified 2015]

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