The enzyme from Saccharomyces cerevisiae specifically methylates guanine9 [1,2]. The bifunctional enzyme from Thermococcus kodakaraensis also catalyses the methylation of adenine9 in tRNA (cf. EC 184.108.40.206, tRNA (adenine9-N1)-methyltransferase) .
Kempenaers, M., Roovers, M., Oudjama, Y., Tkaczuk, K.L., Bujnicki, J.M. and Droogmans, L. New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA. Nucleic Acids Res.38 (2010) 6533–6543. [DOI] [PMID: 20525789]
Jackman, J.E., Montange, R.K., Malik, H.S. and Phizicky, E.M. Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9. RNA9 (2003) 574–585. [DOI] [PMID: 12702816]
[EC 220.127.116.11 created 2011 (EC 18.104.22.168 created 1971, part transferred 2011 to EC 22.214.171.124)]