The enzyme from Sulfolobus acidocaldarius specifically methylates adenine9 in tRNA . The bifunctional enzyme from Thermococcus kodakaraensis also catalyses the methylation of guanine9 in tRNA (cf. EC 22.214.171.124, tRNA (guanine9-N1)-methyltransferase).
Kempenaers, M., Roovers, M., Oudjama, Y., Tkaczuk, K.L., Bujnicki, J.M. and Droogmans, L. New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA. Nucleic Acids Res.38 (2010) 6533–6543. [PMID: 20525789]
[EC 126.96.36.199 created 2011 (EC 188.8.131.52 created 1972, part transferred 2011 to EC 184.108.40.206)]