The enzyme from Sulfolobus acidocaldarius specifically methylates adenine9 in tRNA . The bifunctional enzyme from Thermococcus kodakaraensis also catalyses the methylation of guanine9 in tRNA (cf. EC 220.127.116.11, tRNA (guanine9-N1)-methyltransferase).
Kempenaers, M., Roovers, M., Oudjama, Y., Tkaczuk, K.L., Bujnicki, J.M. and Droogmans, L. New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA. Nucleic Acids Res.38 (2010) 6533–6543. [DOI] [PMID: 20525789]
[EC 18.104.22.168 created 2011 (EC 22.214.171.124 created 1972, part transferred 2011 to EC 126.96.36.199)]