The Enzyme Database

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Accepted name: putidaredoxin—NAD+ reductase
Reaction: reduced putidaredoxin + NAD+ = oxidized putidaredoxin + NADH + H+
For diagram of camphor catabolism, click here
Other name(s): putidaredoxin reductase; camA (gene name)
Systematic name: putidaredoxin:NAD+ oxidoreductase
Comments: Requires FAD. The enzyme from Pseudomonas putida reduces putidaredoxin. It contains a [2Fe-2S] cluster. Involved in the camphor monooxygenase system (see EC, camphor 5-monooxygenase).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
1.  Roome, P.W., Jr., Philley, J.C. and Peterson, J.A. Purification and properties of putidaredoxin reductase. J. Biol. Chem. 258 (1983) 2593–2598. [PMID: 6401738]
2.  Koga, H., Yamaguchi, E., Matsunaga, K., Aramaki, H. and Horiuchi, T. Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida. J. Biochem. 106 (1989) 831–836. [PMID: 2613690]
3.  Peterson, J.A., Lorence, M.C. and Amarneh, B. Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins. J. Biol. Chem. 265 (1990) 6066–6073. [PMID: 2180940]
4.  Sevrioukova, I.F. and Poulos, T.L. Putidaredoxin reductase, a new function for an old protein. J. Biol. Chem. 277 (2002) 25831–25839. [PMID: 12011076]
5.  Sevrioukova, I.F., Garcia, C., Li, H., Bhaskar, B. and Poulos, T.L. Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida. J. Mol. Biol. 333 (2003) 377–392. [PMID: 14529624]
6.  Sevrioukova, I.F., Li, H. and Poulos, T.L. Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase. J. Mol. Biol. 336 (2004) 889–902. [PMID: 15095867]
7.  Smith, N., Mayhew, M., Holden, M.J., Kelly, H., Robinson, H., Heroux, A., Vilker, V.L. and Gallagher, D.T. Structure of C73G putidaredoxin from Pseudomonas putida. Acta Crystallogr. D Biol. Crystallogr. 60 (2004) 816–822. [PMID: 15103126]
[EC created 2012]

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