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Your query returned 6 entries. Printable version
EC | 1.14.13.197 | ||||||
Transferred entry: | dihydromonacolin L hydroxylase. Now EC 1.14.14.124, dihydromonacolin L hydroxylase | ||||||
EC | 1.14.13.198 | ||||||
Transferred entry: | monacolin L hydroxylase. Now EC 1.14.14.125, monacolin L hydroxylase | ||||||
EC | 1.14.14.124 | ||||||
Accepted name: | dihydromonacolin L hydroxylase | ||||||
Reaction: | dihydromonacolin L acid + O2 + [reduced NADPH—hemoprotein reductase] = monacolin L acid + [oxidized NADPH—hemoprotein reductase] + 2 H2O (overall reaction) (1a) dihydromonacolin L acid + O2 + [reduced NADPH—hemoprotein reductase] = 3α-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH—hemoprotein reductase] + H2O (1b) 3α-hydroxy-3,5-dihydromonacolin L acid = monacolin L acid + H2O (spontaneous) |
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For diagram of lovastatin biosynthesis, click here | |||||||
Glossary: | dihydromonacolin L acid = (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate monacolin L acid = (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoate 3α-hydroxy-3,5-dihydromonacolin L = (3R,5R)-7-[(1R,2R,3S,6R,8aR)-3-hydroxy-2,6-dimethyl-1,2,3,5,6,7,8,8a-octahydronaphthalen-1-yl]-3,5-dihydroxyheptanoate |
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Other name(s): | LovA (ambiguous) | ||||||
Systematic name: | dihydromonacolin L acid,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating) | ||||||
Comments: | A cytochrome P-450 (heme-thiolate) protein. The dehydration of 3α-hydroxy-3,5-dihydromonacolin L acid is believed to be spontaneous [1,2]. The enzyme from fungi also catalyses the reaction of EC 1.14.14.125, monacolin L hydroxylase [3]. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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EC | 1.14.14.125 | ||||||
Accepted name: | monacolin L hydroxylase | ||||||
Reaction: | monacolin L acid + O2 + [reduced NADPH—hemoprotein reductase] = monacolin J acid + [oxidized NADPH—hemoprotein reductase] + H2O | ||||||
For diagram of lovastatin biosynthesis, click here | |||||||
Glossary: | monacolin L acid = (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid monacolin J acid = (3R,5R)-7-[(1S,2S,6R,8S,8aR)-8-hydroxy-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid |
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Other name(s): | LovA (ambiguous) | ||||||
Systematic name: | monacolin L acid,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (8-hydroxylating) | ||||||
Comments: | A cytochrome P-450 (heme-thiolate) protein. The enzyme from fungi also catalyses the reaction of EC 1.14.14.124, dihydromonacolin L hydroxylase. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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EC | 2.3.1.161 | ||||||
Accepted name: | lovastatin nonaketide synthase | ||||||
Reaction: | 9 malonyl-CoA + 11 NADPH + 10 H+ + S-adenosyl-L-methionine + holo-[lovastatin nonaketide synthase] = dihydromonacolin L-[lovastatin nonaketide synthase] + 9 CoA + 9 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O | ||||||
For diagram of polyketides biosynthesis, click here | |||||||
Glossary: | dihydromonacolin L acid = (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]-3,5-dihydroxyheptanoate | ||||||
Other name(s): | LNKS; LovB; LovC; acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl- and enoyl-reducing, thioester-hydrolysing) | ||||||
Systematic name: | acyl-CoA:malonyl-CoA C-acyltransferase (dihydromonacolin L acid-forming) | ||||||
Comments: | This fungal enzyme system comprises a multi-functional polyketide synthase (PKS) and an enoyl reductase. The PKS catalyses many of the chain building reactions of EC 2.3.1.85, fatty-acid synthase system, as well as a reductive methylation and a Diels-Alder reaction, while the reductase is responsible for three enoyl reductions that are necessary for dihydromonacolin L acid production. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 235426-97-8 | ||||||
References: |
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EC | 3.1.2.31 | ||||||
Accepted name: | dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase | ||||||
Reaction: | dihydromonacolin L-[lovastatin nonaketide synthase] + H2O = holo-[lovastatin nonaketide synthase] + dihydromonacolin L acid | ||||||
For diagram of lovastatin biosynthesis, click here | |||||||
Glossary: | dihydromonacolin L acid = (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]-3,5-dihydroxyheptanoate | ||||||
Other name(s): | LovG | ||||||
Systematic name: | dihydromonacolin L-[lovastatin nonaketide synthase] hydrolase | ||||||
Comments: | Dihydromonacolin L acid is synthesized while bound to an acyl-carrier protein domain of the lovastatin nonaketide synthase (EC 2.3.1.161). Since that enzyme lacks a thioesterase domain, release of the dihydromonacolin L acid moiety from the polyketide synthase requires this dedicated enzyme. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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