For diagram of methanofuran biosynthesis, click here
mfnD (gene name)
tyramine:L-glutamate γ-ligase (ADP-forming)
The enzyme, which has been characterized from the archaea Methanocaldococcus fervens, participates in the biosynthesis of the cofactor methanofuran. Requires a divalent cation for activity, with Mn2+ giving the highest activity, followed by Mg2+, Co2+, Zn2+, and Fe2+.