The enzyme, characterized from the bacterium Pseudomonas alcaligenes NCIMB 9867, catalyses the hydrolysis of 3-maleylpyruvate, the ring-cleavage product of gentisate. The enzyme can also act on a number of maleylpyruvate derivatives, such as (2E)-2-methyl-4,6-dioxohept-2-enedioate and (2E)-3-methyl-4,6-dioxohept-2-enedioate. Activated by Mn2+. May be identical to EC 188.8.131.52, acylpyruvate hydrolase.
Hopper, D.J., Chapman, P.J. and Dagley, S. The enzymic degradation of alkyl-substituted gentisates, maleates and malates. Biochem. J.122 (1971) 29–40. [PMID: 5124802]
Bayly, R.C., Chapman, P.J., Dagley, S. and Di Berardino, D. Purification and some properties of maleylpyruvate hydrolase and fumarylpyruvate hydrolase from Pseudomonas alcaligenes. J. Bacteriol.143 (1980) 70–77. [PMID: 7400101]
Liu, K., Liu, T.T. and Zhou, N.Y. HbzF catalyzes direct hydrolysis of maleylpyruvate in the gentisate pathway of Pseudomonas alcaligenes NCIMB 9867. Appl. Environ. Microbiol.79 (2013) 1044–1047. [PMID: 23204427]