The Enzyme Database

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EC 3.2.1.200     
Accepted name: exo-chitinase (non-reducing end)
Reaction: Hydrolysis of N,N′-diacetylchitobiose from the non-reducing end of chitin and chitodextrins.
Other name(s): chiB (gene name)
Systematic name: (1→4)-2-acetamido-2-deoxy-β-D-glucan diacetylchitobiohydrolase (non-reducing end)
Comments: The enzyme hydrolyses the second glycosidic (1→4) linkage from non-reducing ends of chitin and chitodextrin molecules, liberating N,N′-diacetylchitobiose disaccharides. cf. EC 3.2.1.201, exo-chitinase (reducing end).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Tanaka, T., Fukui, T. and Imanaka, T. Different cleavage specificities of the dual catalytic domains in chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J. Biol. Chem. 276 (2001) 35629–35635. [PMID: 11468293]
2.  Hult, E.L., Katouno, F., Uchiyama, T., Watanabe, T. and Sugiyama, J. Molecular directionality in crystalline β-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170. Biochem. J. 388 (2005) 851–856. [PMID: 15717865]
3.  Ohnuma, T., Numata, T., Osawa, T., Mizuhara, M., Lampela, O., Juffer, A.H., Skriver, K. and Fukamizo, T. A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta 234 (2011) 123–137. [PMID: 21390509]
4.  Gutierrez-Roman, M.I., Dunn, M.F., Tinoco-Valencia, R., Holguin-Melendez, F., Huerta-Palacios, G. and Guillen-Navarro, K. Potentiation of the synergistic activities of chitinases ChiA, ChiB and ChiC from Serratia marcescens CFFSUR-B2 by chitobiase (Chb) and chitin binding protein (CBP). World J Microbiol Biotechnol 30 (2014) 33–42. [PMID: 23824666]
[EC 3.2.1.200 created 2017]
 
 


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