The enzyme acetylates the wobble base cytidine34 of the CAU anticodon of elongation-specific tRNAMet. Escherichia coli TmcA strictly discriminates elongator tRNAMet from tRNAIle, which is structurally similar and has the same anticodon loop, mainly by recognizing the C27-G43 pair in the anticodon stem. The enzyme can use GTP in place of ATP for formation of N4-acetylcytidine .
Ikeuchi, Y., Kitahara, K. and Suzuki, T. The RNA acetyltransferase driven by ATP hydrolysis synthesizes N4-acetylcytidine of tRNA anticodon. EMBO J.27 (2008) 2194–2203. [PMID: 18668122]
Chimnaronk, S., Suzuki, T., Manita, T., Ikeuchi, Y., Yao, M., Suzuki, T. and Tanaka, I. RNA helicase module in an acetyltransferase that modifies a specific tRNA anticodon. EMBO J.28 (2009) 1362–1373. [PMID: 19322199]