The Enzyme Database

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Accepted name: isopenicillin-N N-acyltransferase
Reaction: phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G + L-2-aminohexanedioate
For diagram of penicillin biosynthesis and metabolism, click here
Other name(s): acyl-coenzyme A:isopenicillin N acyltransferase; isopenicillin N:acyl-CoA: acyltransferase
Systematic name: acyl-CoA:isopenicillin N N-acyltransferase
Comments: Proceeds by a two stage mechanism via 6-aminopenicillanic acid. Different from EC, penicillin amidase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 54576-90-8
1.  Tobin, M.B., Fleming, M.D., Skatrud, P.L. and Miller, J.R. Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli. J. Bacteriol. 172 (1990) 5908–5914. [PMID: 2120195]
2.  Aplin, R.T., Baldwin, J.E., Roach, P.L., Robinson, C.V. and Schofield, C.J. Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry. Biochem. J. 294 (1993) 357–363. [PMID: 8396910]
[EC created 2002]

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