EC |
2.3.1.164 |
Accepted name: |
isopenicillin-N N-acyltransferase |
Reaction: |
phenylacetyl-CoA + isopenicillin N + H2O = CoA + penicillin G + L-2-aminohexanedioate |
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For diagram of penicillin biosynthesis and metabolism, click here |
Other name(s): |
acyl-coenzyme A:isopenicillin N acyltransferase; isopenicillin N:acyl-CoA: acyltransferase |
Systematic name: |
acyl-CoA:isopenicillin N N-acyltransferase |
Comments: |
Proceeds by a two stage mechanism via 6-aminopenicillanic acid. Different from EC 3.5.1.11, penicillin amidase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 54576-90-8 |
References: |
1. |
Tobin, M.B., Fleming, M.D., Skatrud, P.L. and Miller, J.R. Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli. J. Bacteriol. 172 (1990) 5908–5914. [DOI] [PMID: 2120195] |
2. |
Aplin, R.T., Baldwin, J.E., Roach, P.L., Robinson, C.V. and Schofield, C.J. Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry. Biochem. J. 294 (1993) 357–363. [PMID: 8396910] |
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[EC 2.3.1.164 created 2002] |
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