The enzyme efficiently methylates guanine966 of the assembled 30S subunits in vitro. Protein-free 16S rRNA is not a substrate for RsmD . The enzyme specifically methylates guanine966 at N2 in 16S rRNA.
Lesnyak, D.V., Osipiuk, J., Skarina, T., Sergiev, P.V., Bogdanov, A.A., Edwards, A., Savchenko, A., Joachimiak, A. and Dontsova, O.A. Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure. J. Biol. Chem.282 (2007) 5880–5887. [PMID: 17189261]
[EC 126.96.36.199 created 1976 as EC 188.8.131.52, part transferred 2010 to EC 184.108.40.206]