The Enzyme Database

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Accepted name: azobenzene reductase
Reaction: N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ = 4-(dimethylamino)azobenzene + 2 NADPH + 2 H+
Glossary: 4-(dimethylamino)azobenzene = Methyl Yellow
Other name(s): new coccine (NC)-reductase; NC-reductase; azo-dye reductase; orange II azoreductase; NAD(P)H:1-(4′-sulfophenylazo)-2-naphthol oxidoreductase; orange I azoreductase; azo reductase; azoreductase; nicotinamide adenine dinucleotide (phosphate) azoreductase; NADPH2-dependent azoreductase; dimethylaminobenzene reductase; p-dimethylaminoazobenzene azoreductase; dibromopropylaminophenylazobenzoic azoreductase; N,N-dimethyl-4-phenylazoaniline azoreductase; p-aminoazobenzene reductase; methyl red azoreductase; NADPH2:4-(dimethylamino)azobenzene oxidoreductase
Systematic name: N,N-dimethyl-1,4-phenylenediamine, aniline:NADP+ oxidoreductase
Comments: The reaction occurs in the reverse direction to that shown above. Other azo dyes, such as Methyl Red, Rocceline, Solar Orange and Sumifix Black B can also be reduced [2].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, UM-BBD, CAS registry number: 9029-31-6
1.  Mueller, G.C. and Miller, J.A. The reductive cleavage of 4-dimethylaminoazobenzene by rat liver: the intracellular distribution of the enzyme system and its requirements for triphosphopyridine nucleotide. J. Biol. Chem. 180 (1949) 1125–1136.
2.  Suzuki, Y., Yoda, T., Ruhul, A. and Sugiura, W. Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J. Biol. Chem. 276 (2001) 9059–9065. [PMID: 11134015]
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