ExplorEnz: EC 1.4.2.2

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1.4.2.2

Accepted name:

nicotine dehydrogenase

Reaction:

(S)-nicotine + 2 ferricytochrome c = N-methylmyosmine + 2 ferrocytochrome c + 2 H⁺

Glossary:

(S)-nicotine = 3-[(S)-1-methylpyrrolidin-2-yl]pyridine
N-methylmyosamine = 3-(1-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridine

Other name(s):

nicA2 (gene name)

Systematic name:

(S)-nicotine:cytochrome c oxidoreductase (N-methylmimosine-forming)

Comments:

The enzyme, characterized from the bacterium Pseudomonas putida S16, contains an FAD cofactor and belongs to the flavin-containing amine oxidase family. The enzyme from this bacterium is specific for the c-type cytochrome CycN. The product undergoes spontaneous hydrolysis to form pseudooxynicotine.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Tang, H., Wang, L., Wang, W., Yu, H., Zhang, K., Yao, Y. and Xu, P. Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas. PLoS Genet. 9:e1003923 (2013). [DOI] [PMID: 24204321]
2.	Dulchavsky, M., Clark, C.T., Bardwell, J.CA. and Stull, F. A cytochrome c is the natural electron acceptor for nicotine oxidoreductase. Nat. Chem. Biol. 17 (2021) 344–350. [DOI] [PMID: 33432238]

[EC 1.4.2.2 created 2022]