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Your query returned 2 entries. Printable version
EC | 3.3.2.9 | ||||||||||||||||||||
Accepted name: | microsomal epoxide hydrolase | ||||||||||||||||||||
Reaction: | (1) cis-stilbene oxide + H2O = (1R,2R)-1,2-diphenylethane-1,2-diol (2) 1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-({[(4-methoxyphenyl)methyl](methyl)amino}methyl)-2-methylpropane-1,3-diol |
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Glossary: | oxirane = ethylene oxide = a 3-membered oxygen-containing ring oxetane = 1,3-propylene oxide = a 4-membered oxygen-containing ring |
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Other name(s): | microsomal oxirane/oxetane hydrolase; epoxide hydratase (ambiguous); microsomal epoxide hydratase (ambiguous); epoxide hydrase; microsomal epoxide hydrase; arene-oxide hydratase (ambiguous); benzo[a]pyrene-4,5-oxide hydratase; benzo(a)pyrene-4,5-epoxide hydratase; aryl epoxide hydrase (ambiguous); cis-epoxide hydrolase; mEH; EPHX1 (gene name) | ||||||||||||||||||||
Systematic name: | cis-stilbene-oxide hydrolase | ||||||||||||||||||||
Comments: | This is a key hepatic enzyme that catalyses the hydrolytic ring opening of oxiranes (epoxides) and oxetanes to give the corresponding diols. The enzyme is involved in the metabolism of numerous substrates including the stereoselective hydrolytic ring opening of 7-oxabicyclo[4.1.0]hepta-2,4-dienes (arene oxides) to the corresponding trans-dihydrodiols. The reaction proceeds via a triad mechanism and involves the formation of an hydroxyalkyl-enzyme intermediate. Five epoxide-hydrolase enzymes have been identified in vertebrates to date: EC 3.3.2.6 (leukotriene-A4 hydrolase), EC 3.3.2.7 (hepoxilin-epoxide hydrolase), EC 3.3.2.9 (microsomal epoxide hydrolase), EC 3.3.2.10 (soluble epoxide hydrolase) and EC 3.3.2.11 (cholesterol-5,6-oxide hydrolase). | ||||||||||||||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||||||||||||||||
References: |
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EC | 4.1.99.13 | ||||||||||||||||||||
Accepted name: | (6-4)DNA photolyase | ||||||||||||||||||||
Reaction: | (6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA) | ||||||||||||||||||||
For diagram click here | |||||||||||||||||||||
Other name(s): | DNA photolyase; H64PRH; NF-10; phr (6-4); PL-(6-4); OtCPF1; (6-4) PHR; At64PHR | ||||||||||||||||||||
Systematic name: | (6-4) photoproduct pyrimidine-lyase | ||||||||||||||||||||
Comments: | A flavoprotein (FAD). The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases. This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH-) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases [2]. | ||||||||||||||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37290-70-3 | ||||||||||||||||||||
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