The Enzyme Database

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EC 3.2.1.171     
Accepted name: rhamnogalacturonan hydrolase
Reaction: Endohydrolysis of α-D-GalA-(1→2)-α-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with β-D-GalA at the reducing end.
Other name(s): rhamnogalacturonase A; RGase A; RG-hydrolase
Systematic name: rhamnogalacturonan α-D-GalA-(1→2)-α-L-Rha hydrolase
Comments: The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB
References:
1.  Petersen, T.N., Kauppinen, S. and Larsen, S. The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix. Structure 5 (1997) 533–544. [DOI] [PMID: 9115442]
2.  Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus. J. Biol. Chem. 269 (1994) 29182–29189. [PMID: 7961884]
3.  Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase. Glycobiology 5 (1995) 783–789. [DOI] [PMID: 8720076]
4.  Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus. Acta Crystallogr. D Biol. Crystallogr. 53 (1997) 105–107. [DOI] [PMID: 15299976]
5.  Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus. Biochem. Biophys. Res. Commun. 242 (1998) 552–559. [DOI] [PMID: 9464254]
[EC 3.2.1.171 created 2011]
 
 


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