ExplorEnz: EC 2.7.7.87

Your query returned 1 entry. Printable version

2.7.7.87

Accepted name:

L-threonylcarbamoyladenylate synthase

Reaction:

L-threonine + ATP + HCO₃^- = L-threonylcarbamoyladenylate + diphosphate + H₂O

For diagram of N⁶-L-Threonylcarbamoyladenosine³⁷ modified tRNA biosynthesis, click here

Other name(s):

yrdC (gene name); Sua5; ywlC (gene name); ATP:L-threonyl,bicarbonate adenylyltransferase

Systematic name:

ATP:L-threonyl,HCO₃^- adenylyltransferase

Comments:

The enzyme is involved in the synthesis of N⁶-threonylcarbamoyladenosine³⁷ in tRNAs, with the anticodon NNU, i.e. tRNA^Ile, tRNA^Thr, tRNA^Asn, tRNA^Lys, tRNA^Ser and tRNA^Arg [6].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	El Yacoubi, B., Lyons, B., Cruz, Y., Reddy, R., Nordin, B., Agnelli, F., Williamson, J.R., Schimmel, P., Swairjo, M.A. and de Crecy-Lagard, V. The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA. Nucleic Acids Res. 37 (2009) 2894–2909. [DOI] [PMID: 19287007]
2.	Harris, K.A., Jones, V., Bilbille, Y., Swairjo, M.A. and Agris, P.F. YrdC exhibits properties expected of a subunit for a tRNA threonylcarbamoyl transferase. RNA 17 (2011) 1678–1687. [DOI] [PMID: 21775474]
3.	Kuratani, M., Kasai, T., Akasaka, R., Higashijima, K., Terada, T., Kigawa, T., Shinkai, A., Bessho, Y. and Yokoyama, S. Crystal structure of Sulfolobus tokodaii Sua5 complexed with L-threonine and AMPPNP. Proteins 79 (2011) 2065–2075. [DOI] [PMID: 21538543]
4.	Lauhon, C.T. Mechanism of N⁶-threonylcarbamoyladenonsine (t⁶A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP. Biochemistry 51 (2012) 8950–8963. [DOI] [PMID: 23072323]
5.	Deutsch, C., El Yacoubi, B., de Crecy-Lagard, V. and Iwata-Reuyl, D. Biosynthesis of threonylcarbamoyl adenosine (t⁶A), a universal tRNA nucleoside. J. Biol. Chem. 287 (2012) 13666–13673. [DOI] [PMID: 22378793]
6.	Perrochia, L., Crozat, E., Hecker, A., Zhang, W., Bareille, J., Collinet, B., van Tilbeurgh, H., Forterre, P. and Basta, T. In vitro biosynthesis of a universal t⁶A tRNA modification in Archaea and Eukarya. Nucleic Acids Res. 41 (2013) 1953–1964. [DOI] [PMID: 23258706]
7.	Wan, L.C.K., Mao, D.Y.L., Neculai, D., Strecker, J., Chiovitti, D., Kurinov, I., Poda, G., Thevakumaran, N., Yuan, F., Szilard, R.K., Lissina, E., Nislow, C., Caudy, A.A., Durocher, D. and Sicheri, F. Reconstitution and characterization of eukaryotic N⁶-threonylcarbamoylation of tRNA using a minimal enzyme system. Nucleic Acids Res. 41 (2013) 6332–6346. [DOI] [PMID: 23620299]

[EC 2.7.7.87 created 2013]