| EC |
2.7.7.50 |
| Accepted name: |
mRNA guanylyltransferase |
| Reaction: |
GTP + a 5′-diphospho-[mRNA] = diphosphate + a 5′-(5′-triphosphoguanosine)-[mRNA] |
| Glossary: |
a 5′-(5′-triphosphoguanosine)-[mRNA] = G(5′)pppPur-mRNA = mRNA containing a guanosine residue linked 5′ through three phosphates to the 5′ position of the terminal residue |
| Other name(s): |
RNGTT (gene name); CEG1 (gene name); mRNA capping enzyme; messenger RNA guanylyltransferase; Protein λ2 |
| Systematic name: |
GTP:mRNA guanylyltransferase |
| Comments: |
The human enzyme is a multi domain protein that also has the activity of EC 3.6.1.74, mRNA 5′-phosphatase. |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 56941-23-2 |
| References: |
| 1. |
Ensinger, M.J., Martin, S.A., Paoletti, E. and Moss, B. Modification of the 5′-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus. Proc. Natl. Acad. Sci. USA 72 (1975) 2525–2529. [DOI] [PMID: 1058472] |
| 2. |
Groner, Y., Gilbao, E. and Aviv, H. Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates. Biochemistry 17 (1978) 977–982. [PMID: 629955] |
| 3. |
Itoh, N., Yamada, H., Kaziro, Y. and Mizumoto, K. Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. Large scale purification, subunit functions, and subcellular localization. J. Biol. Chem. 262 (1987) 1989–1995. [PMID: 3029058] |
| 4. |
Martin, S.A. and Moss, B. Modification of RNA by mRNA guanylyltransferase and mRNA(guanine-7-)methyltransferase from vaccinia virions. J. Biol. Chem. 250 (1975) 9330–9335. [PMID: 1194287] |
| 5. |
Martin, S.A., Paoletti, E. and Moss, B. Purification of mRNA guanylyltransferase and mRNA(guanine-7-)methyltransferase from vaccinia virions. J. Biol. Chem. 250 (1975) 9322–9329. [PMID: 1194286] |
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| [EC 2.7.7.50 created 1981, modified 2021] |
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