ExplorEnz: EC 2.3.1.185

Your query returned 1 entry. Printable version

2.3.1.185

Accepted name:

tropine acyltransferase

Reaction:

an acyl-CoA + tropine = CoA + an O-acyltropine

For diagram of tropane alkaloid biosynthesis, click here and for diagram of tropane alkaloid biosynthesis, click here

Glossary:

tropine = tropan-3α-ol = 3α-hydroxytropane

Other name(s):

tropine:acyl-CoA transferase; acetyl-CoA:tropan-3-ol acyltransferase; tropine acetyltransferase; tropine tigloyltransferase; TAT

Systematic name:

acyl-CoA:tropine O-acyltransferase

Comments:

This enzyme exhibits absolute specificity for the 3α configuration found in tropine (tropan-3α-ol). Pseudotropine (tropan-3β-ol) is not a substrate [3]. (E)-2-methylcrotonoyl-CoA (tigloyl-CoA) and acetyl-CoA are the best substrates.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 138440-79-6, 162535-29-7

References:

1.	Robins, R.J., Bachmann, P., Robinson, T., Rhodes, M.J. and Yamada, Y. The formation of 3α- and 3β-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases. FEBS Lett. 292 (1991) 293–297. [DOI] [PMID: 1959620]
2.	Robins, R.J., Bachmann,P., Peerless, A.C.J. and Rabot, S. Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures. Plant Cell, Tissue Organ Cult. 38 (1994) 241–247.
3.	Boswell, H.D., Dräger, B., McLauchlan, W.R., Portsteffen, A., Robins, D.J., Robins, R.J. and Walton, N.J. Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura. Phytochemistry 52 (1999) 871–878. [DOI] [PMID: 10626376]

[EC 2.3.1.185 created 2008]