ExplorEnz: EC 1.8.1.12

Your query returned 1 entry. Printable version

1.8.1.12

Accepted name:

trypanothione-disulfide reductase

Reaction:

trypanothione + NADP⁺ = trypanothione disulfide + NADPH + H⁺

For diagram of trypanothione biosynthesis, click here

Glossary:

spermidine = N-(3-aminopropyl)butane-1,4-diamine
trypanothione = N¹,N⁸-bis(glutathionyl)spermidine

Other name(s):

trypanothione reductase; NADPH₂:trypanothione oxidoreductase

Systematic name:

trypanothione:NADP⁺ oxidoreductase

Comments:

Trypanothione disulfide is the oxidized form of N¹,N⁸-bis(glutathionyl)-spermidine from the insect-parasitic trypanosomatid Crithidia fasciculata. The enzyme from Crithidia fasciculata is a flavoprotein (FAD), whose activity is dependent on a redox-active cystine at the active centre. (cf. EC 1.8.1.7, glutathione-disulfide reductase)

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 102210-35-5

References:

1.	Shames, S.L., Fairlamb, A.H., Cerami, A. and Walsh, C.T. Purification and characterization of trypanothione reductase from Crithidia fasciculata, a newly discovered member of the family of disulfide-containing flavoprotein reductases. Biochemistry 25 (1986) 3519–3526. [PMID: 3718941]
2.	Marsh, I.R. and Bradley, M. Substrate specificity of trypanothione reductase. Eur. J. Biochem. 243 (1977) 690–694. [DOI] [PMID: 9057833]
3.	Cunningham, M.L. and Fairlamb, A.H. Trypanothione reductase from Leishmania donovani. Purification, characterisation and inhibition by trivalent antimonials. Eur. J. Biochem. 230 (1995) 460–468. [DOI] [PMID: 7607216]

[EC 1.8.1.12 created 1989 as EC 1.6.4.8, transferred 2002 to EC 1.8.1.12]