ExplorEnz: EC 1.5.1.43

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1.5.1.43

Accepted name:

carboxynorspermidine synthase

Reaction:

(1) carboxynorspermidine + H₂O + NADP⁺ = L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H⁺
(2) carboxyspermidine + H₂O + NADP⁺ = L-aspartate 4-semialdehyde + putrescine + NADPH + H⁺

Other name(s):

carboxynorspermidine dehydrogenase; carboxyspermidine dehydrogenase; CASDH; CANSDH; VC1624 (gene name)

Systematic name:

carboxynorspermidine:NADP⁺ oxidoreductase

Comments:

The reaction takes place in the opposite direction. Part of a bacterial polyamine biosynthesis pathway. L-aspartate 4-semialdehyde and propane-1,3-diamine/putrescine form a Schiff base that is reduced to form carboxynorspermidine/carboxyspermidine, respectively [1]. The enzyme from the bacterium Vibrio cholerae is essential for biofilm formation [2]. The enzyme from Campylobacter jejuni only produces carboxyspermidine in vivo even though it also can produce carboxynorspermidine in vitro [3].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc

References:

1.	Nakao, H., Shinoda, S. and Yamamoto, S. Purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine in Vibrio alginolyticus. J. Gen. Microbiol. 137 (1991) 1737–1742. [DOI] [PMID: 1955861]
2.	Lee, J., Sperandio, V., Frantz, D.E., Longgood, J., Camilli, A., Phillips, M.A. and Michael, A.J. An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae. J. Biol. Chem. 284 (2009) 9899–9907. [DOI] [PMID: 19196710]
3.	Hanfrey, C.C., Pearson, B.M., Hazeldine, S., Lee, J., Gaskin, D.J., Woster, P.M., Phillips, M.A. and Michael, A.J. Alternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in human gut microbiota. J. Biol. Chem. 286 (2011) 43301–43312. [DOI] [PMID: 22025614]

[EC 1.5.1.43 created 2012]