||The enzyme, characterized from the bacterium Streptomyces thioluteus, catalyses an early step in the biosynthesis of the antibiotic aureothin. It contains a carboxylate-bridged binuclear non-heme iron cluster. The native electron donor has not been identified, but is likely an iron-sulfur protein. The reaction mechanism involves formation of an extremely stable peroxo intermediate that catalyses three two-electron oxidations via a hydroxylamine and dihydroxylamine intermediates. cf. EC 184.108.40.206, N-[1-(4-aminophenyl)-1,3-dihydroxypropan-2-yl]-2,2-dichloroacetamide N-oxygenase.
||He, J. and Hertweck, C. Biosynthetic origin of the rare nitroaryl moiety of the polyketide antibiotic aureothin: involvement of an unprecedented N-oxygenase. J. Am. Chem. Soc. 126 (2004) 3694–3695. [DOI] [PMID: 15038705]
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||Zocher, G., Winkler, R., Hertweck, C. and Schulz, G.E. Structure and action of the N-oxygenase AurF from Streptomyces thioluteus. J. Mol. Biol. 373 (2007) 65–74. [DOI] [PMID: 17765264]
||Choi, Y.S., Zhang, H., Brunzelle, J.S., Nair, S.K. and Zhao, H. In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis. Proc. Natl. Acad. Sci. USA 105 (2008) 6858–6863. [DOI] [PMID: 18458342]
||Korboukh, V.K., Li, N., Barr, E.W., Bollinger, J.M., Jr. and Krebs, C. A long-lived, substrate-hydroxylating peroxodiiron(III/III) intermediate in the amine oxygenase, AurF, from Streptomyces thioluteus. J. Am. Chem. Soc. 131 (2009) 13608–13609. [DOI] [PMID: 19731912]
||Li, N., Korboukh, V.K., Krebs, C. and Bollinger, J.M., Jr. Four-electron oxidation of p-hydroxylaminobenzoate to p-nitrobenzoate by a peroxodiferric complex in AurF from Streptomyces thioluteus. Proc. Natl. Acad. Sci. USA 107 (2010) 15722–15727. [DOI] [PMID: 20798054]