ExplorEnz: EC 1.14.13.20

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1.14.13.20

Accepted name:

2,4-dichlorophenol 6-monooxygenase

Reaction:

2,4-dichlorophenol + NADPH + H⁺ + O₂ = 3,5-dichlorocatechol + NADP⁺ + H₂O

Other name(s):

2,4-dichlorophenol hydroxylase; 2,4-dichlorophenol monooxygenase

Systematic name:

2,4-dichlorophenol,NADPH:oxygen oxidoreductase (6-hydroxylating)

Comments:

A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and 4-chloro-2-methylphenol; NADH can act instead of NADPH, but more slowly.

Links to other databases:

BRENDA, EAWAG-BBD, EXPASY, GENE, KEGG, MetaCyc, CAS registry number: 82047-82-3

References:

1.	Beadle, C.A. and Smith, A.R.W. The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species. Eur. J. Biochem. 123 (1982) 323–332. [DOI] [PMID: 7075592]
2.	Perkins, E.J., Gordon, M.P., Caceres, O. and Lurquin, P.F. Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4. J. Bacteriol. 172 (1990) 2351–2359. [DOI] [PMID: 2185214]
3.	Radjendirane, V., Bhat, M.A. and Vaidyanathan, C.S. Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia. Arch. Biochem. Biophys. 288 (1991) 169–176. [DOI] [PMID: 1898014]
4.	Ledger, T., Pieper, D.H. and Gonzalez, B. Chlorophenol hydroxylases encoded by plasmid pJP4 differentially contribute to chlorophenoxyacetic acid degradation. Appl. Environ. Microbiol. 72 (2006) 2783–2792. [DOI] [PMID: 16597983]
5.	Lu, Y., Yu, Y., Zhou, R., Sun, W., Dai, C., Wan, P., Zhang, L., Hao, D. and Ren, H. Cloning and characterisation of a novel 2,4-dichlorophenol hydroxylase from a metagenomic library derived from polychlorinated biphenyl-contaminated soil. Biotechnol. Lett. 33 (2011) 1159–1167. [DOI] [PMID: 21293902]

[EC 1.14.13.20 created 1983]