EC |
1.1.3.23 |
Accepted name: |
thiamine oxidase |
Reaction: |
thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2 |
Other name(s): |
thiamin dehydrogenase; thiamine dehydrogenase; thiamin:oxygen 5-oxidoreductase |
Systematic name: |
thiamine:oxygen 5-oxidoreductase |
Comments: |
A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 96779-44-1 |
References: |
1. |
Edmondson, D.E., Kenney, W.C. and Singer, T.P. Structural elucidation and properties of 8α-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and β-cyclopiazonate oxidocyclase. Biochemistry 15 (1976) 2937–2945. [PMID: 8076] |
2. |
Gomez-Moreno, C. and Edmondson, D.E. Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase. Arch. Biochem. Biophys. 239 (1985) 46–52. [DOI] [PMID: 2988447] |
3. |
Neal, R.A. Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2′-methyl-4′-amino-5′-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism. J. Biol. Chem. 245 (1970) 2599–2604. [PMID: 4987737] |
|
[EC 1.1.3.23 created 1984] |
|
|
|
|