Reaction: (+)-germacrene A + NADPH + H+ + O2 = germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O
For diagram click here.
Systematic name: (+)-germacrene-A,NADPH:oxygen oxidoreductase (12-hydroxylating)
Comments: A heme-thiolate protein (P-450).
References:
1. de Kraker, J.W., Franssen, M.C., Dalm, M.C., de Groot, A. and Bouwmeester, H.J. Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene A hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis. Plant Physiol. 125 (2001) 1930-1940. [PMID: 11299372]