EC |
2.5.1.135 |
Accepted name: |
validamine 7-phosphate valienyltransferase |
Reaction: |
GDP-valienol + validamine 7-phosphate = validoxylamine A 7′-phosphate + GDP |
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For diagram of validamycin biosynthesis, click here |
Glossary: |
valienol = (1S,2S,3S,4R)-5-(hydroxymethyl)cyclohex-5-ene-1,2,3,4-tetrol
validamine = (1R,2S,3S,4S,6R)-4-amino-6-(hydroxymethyl)cyclohexane-1,2,3-triol |
Other name(s): |
vldE (gene name); valL (gene name) |
Systematic name: |
GDP-valienol:validamine 7-phosphate valienyltransferase |
Comments: |
The enzyme, characterized from several Streptomyces strains, is involved in the biosynthesis of the antifungal agent validamycin A. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Asamizu, S., Yang, J., Almabruk, K.H. and Mahmud, T. Pseudoglycosyltransferase catalyzes nonglycosidic C-N coupling in validamycin a biosynthesis. J. Am. Chem. Soc. 133 (2011) 12124–12135. [DOI] [PMID: 21766819] |
2. |
Zheng, L., Zhou, X., Zhang, H., Ji, X., Li, L., Huang, L., Bai, L. and Zhang, H. Structural and functional analysis of validoxylamine A 7′-phosphate synthase ValL involved in validamycin A biosynthesis. PLoS One 7:e32033 (2012). [DOI] [PMID: 22384130] |
3. |
Cavalier, M.C., Yim, Y.S., Asamizu, S., Neau, D., Almabruk, K.H., Mahmud, T. and Lee, Y.H. Mechanistic insights into validoxylamine A 7′-phosphate synthesis by VldE using the structure of the entire product complex. PLoS One 7:e44934 (2012). [DOI] [PMID: 23028689] |
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[EC 2.5.1.135 created 2016] |
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EC |
2.7.7.91 |
Accepted name: |
valienol-1-phosphate guanylyltransferase |
Reaction: |
GTP + valienol 1-phosphate = diphosphate + GDP-valienol |
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For diagram of validamycin biosynthesis, click here |
Glossary: |
valienol 1-phosphate = (1S,4R,5S,6R)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl phosphate |
Other name(s): |
vldB (gene name) |
Systematic name: |
GTP:valienol 1-phosphate guanylyltransferase |
Comments: |
The enzyme, characterized from the bacterium Streptomyces hygroscopicus subsp. limoneus, is involved in the biosynthesis of the antifungal agent validamycin A. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Yang, J., Xu, H., Zhang, Y., Bai, L., Deng, Z. and Mahmud, T. Nucleotidylation of unsaturated carbasugar in validamycin biosynthesis. Org. Biomol. Chem. 9 (2011) 438–449. [DOI] [PMID: 20981366] |
2. |
Asamizu, S., Yang, J., Almabruk, K.H. and Mahmud, T. Pseudoglycosyltransferase catalyzes nonglycosidic C-N coupling in validamycin a biosynthesis. J. Am. Chem. Soc. 133 (2011) 12124–12135. [DOI] [PMID: 21766819] |
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[EC 2.7.7.91 created 2016] |
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