| EC |
1.14.15.13 |
| Accepted name: |
pulcherriminic acid synthase |
| Reaction: |
cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O |
| Glossary: |
cyclo(L-leucyl-L-leucyl) = (3S,6S)-3,6-bis(2-methylpropyl)piperazine-2,5-dione
pulcherriminic acid = 2,5-dihydroxy-3,6-bis(2-methylpropyl)pyrazine bis-N-oxide |
| Other name(s): |
cyclo-L-leucyl-L-leucyl dipeptide oxidase; CYP134A1; CypX (ambiguous) |
| Systematic name: |
cyclo(L-leucyl-L-leucyl),reduced-ferredoxin:oxygen oxidoreductase (N-hydroxylating,aromatizing) |
| Comments: |
A heme-thiolate (P-450) enzyme from the bacterium Bacillus subtilis. The order of events during the overall reaction is unknown. Pulcherrimic acid spontaneously forms an iron chelate with Fe(3+) to form the red pigment pulcherrimin [2]. |
| References: |
| 1. |
MacDonald, J.C. Biosynthesis of pulcherriminic acid. Biochem. J. 96 (1965) 533–538. [PMID: 5837792] |
| 2. |
Cryle, M.J., Bell, S.G. and Schlichting, I. Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase. Biochemistry 49 (2010) 7282–7296. [PMID: 20690619] |
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| [EC 1.14.15.13 created 2013] |
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