| EC |
1.13.12.18 |
| Accepted name: |
dinoflagellate luciferase |
| Reaction: |
dinoflagellate luciferin + O2 = oxidized dinoflagellate luciferin + H2O + hν |
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For diagram of reaction, click here |
| Glossary: |
dinoflagellate luciferin = (1S,2S,3S)-1-carboxy-3-(2-carboxyethyl)-12-ethyl-2,8,13,18-tetramethyl-17-vinyl-1,2,3,21-tetrahydro-5,7-ethanobilene-a-19(16H),52-dione |
| Other name(s): |
(dinoflagellate luciferin) luciferase; Gonyaulax luciferase |
| Systematic name: |
dinoflagellate-luciferin:oxygen 132-oxidoreductase |
| Comments: |
A luciferase from dinoflagellates such as Gonyaulax polyedra, Lingulodinium polyedrum, Noctiluca scintillans, and Pyrocystis lunula. It is a single protein with three luciferase domains. The luciferin is strongly bound by a luciferin binding protein above a pH of 7. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 303183-71-3 |
| References: |
| 1. |
Dunlap, J.C. and Hastings, J.W. The biological clock in Gonyaulax controls luciferase activity by regulating turnover. J. Biol. Chem. 256 (1981) 10509–10518. [PMID: 7197271] |
| 2. |
Morse, D., Pappenheimer, A.M., Jr. and Hastings, J.W. Role of a luciferin-binding protein in the circadian bioluminescent reaction of Gonyaulax polyedra. J. Biol. Chem. 264 (1989) 11822–11826. [PMID: 2745419] |
| 3. |
Bae, Y.M. and Hastings, J.W. Cloning, sequencing and expression of dinoflagellate luciferase DNA from a marine alga, Gonyaulax polyedra. Biochim. Biophys. Acta 1219 (1994) 449–456. [DOI] [PMID: 7918642] |
| 4. |
Li, L. Gonyaulax luciferase: gene structure, protein expression, and purification from recombinant sources. Methods Enzymol. 305 (2000) 249–258. [PMID: 10812605] |
| 5. |
Morse, D. and Mittag, M. Dinoflagellate luciferin-binding protein. Methods Enzymol. 305 (2000) 258–276. [PMID: 10812606] |
| 6. |
Schultz, L.W., Liu, L., Cegielski, M. and Hastings, J.W. Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole. Proc. Natl. Acad. Sci. USA 102 (2005) 1378–1383. [DOI] [PMID: 15665092] |
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| [EC 1.13.12.18 created 2011] |
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