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1.14.13.175

Transferred entry:

aflatoxin B synthase. Now EC 1.14.14.117, aflatoxin B synthase

[EC 1.14.13.175 created 2013, deleted 2018]

1.14.14.117

Accepted name:

aflatoxin B synthase

Reaction:

(1) 8-O-methylsterigmatocystin + 2 [reduced NADPH—hemoprotein reductase] + 2 O₂ = aflatoxin B₁ + 2 [oxidized NADPH—hemoprotein reductase] + H₂O + methanol + CO₂
(2) 8-O-methyldihydrosterigmatocystin + 2 [reduced NADPH—hemoprotein reductase] + 2 O₂ = aflatoxin B₂ + 2 [oxidized NADPH—hemoprotein reductase] + H₂O + methanol + CO₂

For diagram of aflatoxin biosynthesis (part 4), click here

Glossary:

aflatoxin B₁ = (6aR,9aS)-4-methoxy-2,3,6a,9a-tetrahydrocyclopenta[c]furo[3′,2′:4,5]furo[2,3-h][1]benzopyran-1,11-dione
aflatoxin B₂ = (6aR,9aS)-4-methoxy-2,3,6a,8,9,9a-hexahydrocyclopenta[c]furo[3′,2′:4,5]furo[2,3-h][1]benzopyran-1,11-dione
8-O-methylsterigmatocystin = 6,8-dimethoxy-3a,12c-dihydrofuro[3′,2′:4,5]furo[2,3-c]xanthen-7-one
8-O-methyldihydrosterigmatocystin = 6,8-dimethoxy-1,2,3a,12c-tetrahydrofuro[3′,2′:4,5]furo[2,3-c]xanthen-7-one

Other name(s):

ordA (gene name)

Systematic name:

8-O-methylsterigmatocystin,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (aflatoxin-B-forming)

Comments:

A cytochrome P-450 (heme-thiolate) protein. Isolated from the mold Aspergillus parasiticus.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Bhatnagar, D., Cleveland, T.E. and Kingston, D.G. Enzymological evidence for separate pathways for aflatoxin B₁ and B₂ biosynthesis. Biochemistry 30 (1991) 4343–4350. [PMID: 1902378]
2.	Yu, J., Chang, P.K., Ehrlich, K.C., Cary, J.W., Montalbano, B., Dyer, J.M., Bhatnagar, D. and Cleveland, T.E. Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2. Appl. Environ. Microbiol. 64 (1998) 4834–4841. [PMID: 9835571]
3.	Udwary, D.W., Casillas, L. K. and Townsend, C.A. Synthesis of 11-hydroxyl O-methylsterigmatocystin and the role of a cytochrome P-450 in the final step of aflatoxin biosynthesis. J. Am. Chem. Soc. 124 (2002) 5294–5303. [DOI] [PMID: 11996570]

[EC 1.14.14.117 created 2013 as EC 1.14.13.175, transferred 2018 to EC 1.14.14.117]