EC |
2.6.99.3 |
Accepted name: |
O-ureido-L-serine synthase |
Reaction: |
O-acetyl-L-serine + hydroxyurea = O-ureido-L-serine + acetate |
Glossary: |
O-ureido-L-serine = (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
O-acetyl-L-serine = O3-acetyl-L-serine = (2S)-3-acetyloxy-2-aminopropanoic acid
|
Other name(s): |
dcsD (gene name) |
Systematic name: |
O-acetyl-L-serine:hydroxyurea 2-amino-2-carboxyethyltransferase |
Comments: |
The enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species. Also catalyses EC 2.5.1.47, cysteine synthase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Kumagai, T., Koyama, Y., Oda, K., Noda, M., Matoba, Y. and Sugiyama, M. Molecular cloning and heterologous expression of a biosynthetic gene cluster for the antitubercular agent D-cycloserine produced by Streptomyces lavendulae. Antimicrob. Agents Chemother. 54 (2010) 1132–1139. [DOI] [PMID: 20086163] |
2. |
Uda, N., Matoba, Y., Kumagai, T., Oda, K., Noda, M. and Sugiyama, M. Establishment of an in vitro D-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway. Antimicrob. Agents Chemother. 57 (2013) 2603–2612. [DOI] [PMID: 23529730] |
|
[EC 2.6.99.3 created 2013] |
|
|
|
|
EC |
5.1.1.19 |
Accepted name: |
O-ureido-serine racemase |
Reaction: |
O-ureido-L-serine = O-ureido-D-serine |
Glossary: |
O-ureido-L-serine = (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate
O-ureido-D-serine = (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate |
Other name(s): |
dcsC (gene name) |
Systematic name: |
(2S)-2-amino-3-[(carbamoylamino)oxy]propanoate 2-epimerase |
Comments: |
The enzyme employs a two-base mechanism, with a thiolate-thiol pair in the active site. It participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Kumagai, T., Koyama, Y., Oda, K., Noda, M., Matoba, Y. and Sugiyama, M. Molecular cloning and heterologous expression of a biosynthetic gene cluster for the antitubercular agent D-cycloserine produced by Streptomyces lavendulae. Antimicrob. Agents Chemother. 54 (2010) 1132–1139. [DOI] [PMID: 20086163] |
2. |
Dietrich, D., van Belkum, M.J. and Vederas, J.C. Characterization of DcsC, a PLP-independent racemase involved in the biosynthesis of D-cycloserine. Org. Biomol. Chem. 10 (2012) 2248–2254. [DOI] [PMID: 22307920] |
|
[EC 5.1.1.19 created 2013] |
|
|
|
|
EC |
6.3.3.5 |
Accepted name: |
O-ureido-D-serine cyclo-ligase |
Reaction: |
O-ureido-D-serine + ATP + H2O = D-cycloserine + CO2 + NH3 + ADP + phosphate |
Glossary: |
O-ureido-D-serine = (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate |
Other name(s): |
dcsG (gene name) |
Systematic name: |
O-ureido-D-serine cyclo-ligase (D-cycloserine-forming) |
Comments: |
The enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Kumagai, T., Koyama, Y., Oda, K., Noda, M., Matoba, Y. and Sugiyama, M. Molecular cloning and heterologous expression of a biosynthetic gene cluster for the antitubercular agent D-cycloserine produced by Streptomyces lavendulae. Antimicrob. Agents Chemother. 54 (2010) 1132–1139. [DOI] [PMID: 20086163] |
2. |
Uda, N., Matoba, Y., Kumagai, T., Oda, K., Noda, M. and Sugiyama, M. Establishment of an in vitro D-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway. Antimicrob. Agents Chemother. 57 (2013) 2603–2612. [DOI] [PMID: 23529730] |
|
[EC 6.3.3.5 created 2013] |
|
|
|
|