EC |
2.1.2.14 |
Accepted name: |
GDP-perosamine N-formyltransferase |
Reaction: |
10-formyltetrahydrofolate + GDP-α-D-perosamine = tetrahydrofolate + GDP-N-formyl-α-D-perosamine |
Glossary: |
GDP-α-D-perosamine = GDP-4-amino-4,6-dideoxy-α-D-mannose |
Other name(s): |
wbkC (gene name) |
Systematic name: |
10-formyltetrahydrofolate:GDP-α-D-perosamine N-formyltransferase |
Comments: |
The enzyme, characterized from the bacterium Brucella melitensis, synthesizes a building block of the O antigen produced by Brucella species. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Godfroid, F., Cloeckaert, A., Taminiau, B., Danese, I., Tibor, A., de Bolle, X., Mertens, P. and Letesson, J.J. Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk). Res. Microbiol. 151 (2000) 655–668. [DOI] [PMID: 11081580] |
2. |
Riegert, A.S., Chantigian, D.P., Thoden, J.B., Tipton, P.A. and Holden, H.M. Biochemical characterization of WbkC, an N-formyltransferase from Brucella melitensis. Biochemistry 56 (2017) 3657–3668. [DOI] [PMID: 28636341] |
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[EC 2.1.2.14 created 2021] |
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EC |
2.3.1.227 |
Accepted name: |
GDP-perosamine N-acetyltransferase |
Reaction: |
acetyl-CoA + GDP-4-amino-4,6-dideoxy-α-D-mannose = CoA + GDP-4-acetamido-4,6-dideoxy-α-D-mannose |
Glossary: |
GDP-4-amino-4,6-dideoxy-α-D-mannose = GDP-α-D-perosamine
GDP-4-acetamido-4,6-dideoxy-α-D-mannose = GDP-N-acetyl-α-D-perosamine
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Other name(s): |
perB (gene name); GDP-α-D-perosamine N-acetyltransferase |
Systematic name: |
acetyl-CoA:GDP-4-amino-4,6-dideoxy-α-D-mannose N-acetyltransferase |
Comments: |
D-Perosamine is one of several dideoxy sugars found in the O-antigen component of the outer membrane lipopolysaccharides of Gram-negative bacteria. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Albermann, C. and Beuttler, H. Identification of the GDP-N-acetyl-d-perosamine producing enzymes from Escherichia coli O157:H7. FEBS Lett. 582 (2008) 479–484. [DOI] [PMID: 18201574] |
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[EC 2.3.1.227 created 2013] |
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EC |
2.6.1.102 |
Accepted name: |
GDP-perosamine synthase |
Reaction: |
GDP-α-D-perosamine + 2-oxoglutarate = GDP-4-dehydro-α-D-rhamnose + L-glutamate |
Glossary: |
GDP-α-D-perosamine = GDP-4-amino-4,6-dideoxy-α-D-mannose
GDP-4-dehydro-α-D-rhamnose = GDP-4-dehydro-6-deoxy-α-D-mannose |
Other name(s): |
RfbE; GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase; GDP-perosamine synthetase; PerA; GDP-4-amino-4,6-dideoxy-α-D-mannose:2-oxoglutarate aminotransferase |
Systematic name: |
GDP-α-D-perosamine:2-oxoglutarate aminotransferase |
Comments: |
A pyridoxal 5′-phosphate enzyme. D-Perosamine is one of several dideoxy sugars found in the O-specific polysaccharide of the lipopolysaccharide component of the outer membrane of Gram-negative bacteria. The enzyme catalyses the final step in GDP-α-D-perosamine synthesis. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Albermann, C. and Piepersberg, W. Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine. Glycobiology 11 (2001) 655–661. [DOI] [PMID: 11479276] |
2. |
Zhao, G., Liu, J., Liu, X., Chen, M., Zhang, H. and Wang, P.G. Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro. Biochem. Biophys. Res. Commun. 363 (2007) 525–530. [DOI] [PMID: 17888872] |
3. |
Albermann, C. and Beuttler, H. Identification of the GDP-N-acetyl-d-perosamine producing enzymes from Escherichia coli O157:H7. FEBS Lett. 582 (2008) 479–484. [DOI] [PMID: 18201574] |
4. |
Cook, P.D., Carney, A.E. and Holden, H.M. Accommodation of GDP-linked sugars in the active site of GDP-perosamine synthase. Biochemistry 47 (2008) 10685–10693. [DOI] [PMID: 18795799] |
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[EC 2.6.1.102 created 2013] |
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