|
|
Your query returned 4 entries. 
Printable version
| EC | 1.14.13.112 | ||||
| Transferred entry: | 3-epi-6-deoxocathasterone 23-monooxygenase. Now EC 1.14.14.147, 3-epi-6-deoxocathasterone 23-monooxygenase | ||||
| EC | 1.14.14.147 | ||||
| Accepted name: | 22α-hydroxysteroid 23-monooxygenase | ||||
| Reaction: | (1) 3-epi-6-deoxocathasterone + [reduced NADPH—hemoprotein reductase] + O2 = 6-deoxotyphasterol + [oxidized NADPH—hemoprotein reductase] + H2O (2) (22S,24R)-22-hydroxy-5α-ergostan-3-one + [reduced NADPH—hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH—hemoprotein reductase] + H2O |
||||
| Other name(s): | cytochrome P450 90C1; CYP90D1; CYP90C1; 3-epi-6-deoxocathasterone,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating); 3-epi-6-deoxocathasterone 23-monooxygenase | ||||
| Systematic name: | 22α-hydroxysteroid,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating) | ||||
| Comments: | A cytochrome P-450 (heme-thiolate) protein involved in brassinosteroid biosynthesis in plants. The enzyme has a relaxed substrate specificity, and C-23 hydroxylation can occur at different stages in the pathway. In Arabidopsis thaliana two isozymes, encoded by the CYP90C1 and CYP90D1 genes, have redundant activities. | ||||
| Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||
| References: |
| ||||
| EC | 1.14.14.179 | ||||
| Accepted name: | brassinosteroid 6-oxygenase | ||||
| Reaction: | 6-deoxocastasterone + 2 O2 + 2 [reduced NADPH—hemoprotein reductase] = castasterone + 3 H2O + 2 [oxidized NADPH—hemoprotein reductase] (overall reaction) (1a) 6-deoxocastasterone + O2 + [reduced NADPH—hemoprotein reductase] = 6α-hydroxy-6-deoxocastasterone + H2O + [oxidized NADPH—hemoprotein reductase] (1b) 6α-hydroxy-6-deoxocastasterone + O2 + [reduced NADPH—hemoprotein reductase] = castasterone + 2 H2O + [oxidized NADPH—hemoprotein reductase] |
||||
| For diagram of brassinolide biosynthesis, click here | |||||
| Other name(s): | CYP85A1 (gene name); CYP85A2 (gene name); brassinosteroid 6-oxidase | ||||
| Systematic name: | 6-deoxocastasterone,NADPH—hemoprotein reductase:oxygen 6-oxidoreductase (castasterone-forming) | ||||
| Comments: | This cytochrome P-450 (heme thiolate) plant enzyme catalyses the C-6 hydoxylation of several brassinosteroid biosynthesis intermediates, and the further oxidation of the hydroxyl group to an oxo group. Substrates include 6-deoxocastasterone, 6-deoxotyphasterol, 3-dehydro-6-deoxoteasterone, and 6-deoxoteasterone. The CYP85A2 isozyme of Arabidopsis thaliana (but not the CYP85A1 isozyme) also catalyses the activity of EC 1.14.14.180, brassinolide synthase. | ||||
| Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||
| References: |
| ||||
| EC | 1.14.19.79 | ||||
| Accepted name: | 3β,22α-dihydroxysteroid 3-dehydrogenase | ||||
| Reaction: | (1) (22S)-22-hydroxycampesterol + [reduced NADPH-hemoprotein reductase] + O2 = (22S)-22-hydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O (2) 6-deoxoteasterone + [reduced NADPH-hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + 2 H2O |
||||
| Glossary: | 6-deoxoteasterone = (22R,23R)-5α-campestane-3β,22,23-triol | ||||
| Other name(s): | CYP90A1 (gene name) | ||||
| Systematic name: | 3β,22α-dihydroxysteroid,[reduced NADPH-hemoprotein reductase]:oxygen 3-oxidoreductase | ||||
| Comments: | This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Arabidopsis thaliana, catalyses C-3 dehydrogenation of all 3β-hydroxy brassinosteroid synthesis intermediates with 22-hydroxylated or 22,23-dihydroxylated side chains. | ||||
| Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||
| References: |
| ||||