The Enzyme Database

Your query returned 5 entries.    printer_iconPrintable version



EC 1.1.1.421     
Accepted name: D-apionate oxidoisomerase
Reaction: D-apionate + NAD+ = 3-oxoisoapionate + NADH + H+
Glossary: 3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate
Other name(s): apnO (gene name)
Systematic name: D-apionate:NAD+ oxidoreductase (isomerizing)
Comments: The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. The reaction involves migration of a hydroxymethyl group from position 3 to position 2 and oxidation of the 3-hydroxyl group. Stereospecificity of the product, 3-oxoisoapionate, has not been determined.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142]
[EC 1.1.1.421 created 2019]
 
 
EC 2.7.1.231     
Accepted name: 3-oxoisoapionate kinase
Reaction: ATP + 3-oxoisoapionate = ADP + 3-oxoisoapionate 4-phosphate
Glossary: 3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate
Other name(s): oiaK (gene name)
Systematic name: ATP:3-oxoisoapionate 4-phosphotransferase
Comments: The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. Stereospecificity of the product, 3-oxoisoapionate 4-phosphate, has not been determined.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142]
[EC 2.7.1.231 created 2020]
 
 
EC 3.7.1.28     
Accepted name: 3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase
Reaction: 3-oxoisoapionate 4-phosphate + H2O = glycolate + 3-phospho-D-glycerate
Glossary: 3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate
Other name(s): oiaT (gene name)
Systematic name: 3-oxoisoapionate-4-phosphate transcarboxylase/glycolylhydrolase (3-phospho-D-glycerate-forming)
Comments: The enzyme, which belongs to the RuBisCO-like-protein (RLP) superfamily, has been characterized from several bacterial species. It participates in the degradation of D-apionate. The reaction is initiated by decarboxylation to generate a stabilized enediolate intermediate, with the sequestered CO2 carboxylating the adjacent enediolate carbon atom. The resulting 3-ketose-1-phosphate intermediate is hydrolysed, as in the authentic RuBisCO-catalysed reaction, to generate glycolate and 3-phospho-D-glycerate. Stereospecificity of 3-oxoisoapionate 4-phosphate has not been determined.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142]
[EC 3.7.1.28 created 2021]
 
 
EC 4.1.1.120     
Accepted name: 3-oxoisoapionate decarboxylase
Reaction: 3-oxoisoapionate = L-erythrulose + CO2
Glossary: 3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate
Other name(s): oiaC (gene name)
Systematic name: 3-oxoisoapionate carboxy-lyase
Comments: The enzyme, characterized from several bacterial species, is involved in the degradation of D-apionate. Stereospecificity of 3-oxoisoapionate has not been determined.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142]
[EC 4.1.1.120 created 2020]
 
 
EC 4.1.1.121     
Accepted name: 3-oxoisoapionate-4-phosphate decarboxylase
Reaction: 3-oxoisoapionate 4-phosphate = L-erythrulose 1-phosphate + CO2
Glossary: 3-oxoisoapionate = 2,4-dihydroxy-2-(hydroxymethyl)-3-oxobutanoate
Other name(s): oiaX (gene name)
Systematic name: 3-oxoisoapionate 4-phosphate carboxy-lyase
Comments: The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. It belongs to the RuBisCO-like-protein (RLP) superfamily. Stereospecificity of 3-oxoisoapionate 4-phosphate has not been determined.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Carter, M.S., Zhang, X., Huang, H., Bouvier, J.T., Francisco, B.S., Vetting, M.W., Al-Obaidi, N., Bonanno, J.B., Ghosh, A., Zallot, R.G., Andersen, H.M., Almo, S.C. and Gerlt, J.A. Functional assignment of multiple catabolic pathways for D-apiose. Nat. Chem. Biol. 14 (2018) 696–705. [DOI] [PMID: 29867142]
[EC 4.1.1.121 created 2020]
 
 


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