EC |
2.5.1.132 |
Accepted name: |
3-deoxy-D-glycero-D-galacto-nonulopyranosonate 9-phosphate synthase |
Reaction: |
phosphoenolpyruvate + D-mannose 6-phosphate + H2O = 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate 9-phosphate + phosphate |
Glossary: |
phosphoenolpyruvate = 2-(phosphooxy)-2-propenoate
3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate = Kdn |
Other name(s): |
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate synthase; 2-keto-3-deoxy-D-glycero-D-galacto-9-phosphonononic acid synthase; Kdn 9-P synthase |
Systematic name: |
phosphoenolpyruvate:D-mannose-6-phosphate 1-(2-carboxy-2-oxoethyl)transferase |
Comments: |
The enzyme participates in the biosynthesis of the sialic acid 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate (Kdn). The human sialic acid synthase (EC 2.5.1.57) is also able to catalyse the reaction. Kdn is abundant in extracellular glycoconjugates of lower vertebrates such as fish and amphibians, but is also found in the capsular polysaccharides of bacteria that belong to the Bacteroides genus. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Angata, T., Nakata, D., Matsuda, T., Kitajima, K. and Troy, F.A., 2nd. Biosynthesis of KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid). Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis. J. Biol. Chem. 274 (1999) 22949–22956. [DOI] [PMID: 10438460] |
2. |
Lawrence, S.M., Huddleston, K.A., Pitts, L.R., Nguyen, N., Lee, Y.C., Vann, W.F., Coleman, T.A. and Betenbaugh, M.J. Cloning and expression of the human N-acetylneuraminic acid phosphate synthase gene with 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid biosynthetic ability. J. Biol. Chem. 275 (2000) 17869–17877. [DOI] [PMID: 10749855] |
3. |
Wang, L., Lu, Z., Allen, K.N., Mariano, P.S. and Dunaway-Mariano, D. Human symbiont Bacteroides thetaiotaomicron synthesizes 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Chem. Biol. 15 (2008) 893–897. [DOI] [PMID: 18804026] |
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[EC 2.5.1.132 created 2016] |
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EC |
2.7.7.92 |
Accepted name: |
3-deoxy-D-glycero-D-galacto-nonulopyranosonate cytidylyltransferase |
Reaction: |
CTP + 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate = diphosphate + CMP-3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate |
Systematic name: |
CTP:3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate cytidylyltransferase |
Comments: |
The enzyme is part of the biosynthesis pathway of the sialic acid 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate (Kdn). Kdn is abundant in extracellular glycoconjugates of lower vertebrates such as fish and amphibians, but is also found in the capsular polysaccharides of bacteria that belong to the Bacteroides genus. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Terada, T., Kitazume, S., Kitajima, K., Inoue, S., Ito, F., Troy, F.A. and Inoue, Y. Synthesis of CMP-deaminoneuraminic acid (CMP-KDN) using the CTP:CMP-3-deoxynonulosonate cytidylyltransferase from rainbow trout testis. Identification and characterization of a CMP-KDN synthetase. J. Biol. Chem. 268 (1993) 2640–2648. [PMID: 8381411] |
2. |
Terada, T., Kitajima, K., Inoue, S., Koppert, K., Brossmer, R. and Inoue, Y. Substrate specificity of rainbow trout testis CMP-3-deoxy-D-glycero-D-galacto-nonulosonic acid (CMP-Kdn) synthetase: kinetic studies of the reaction of natural and synthetic analogues of nonulosonic acid catalyzed by CMP-Kdn synthetase. Eur. J. Biochem. 236 (1996) 852–855. [DOI] [PMID: 8665905] |
3. |
Nakata, D., Munster, A.K., Gerardy-Schahn, R., Aoki, N., Matsuda, T. and Kitajima, K. Molecular cloning of a unique CMP-sialic acid synthetase that effectively utilizes both deaminoneuraminic acid (KDN) and N-acetylneuraminic acid (Neu5Ac) as substrates. Glycobiology 11 (2001) 685–692. [DOI] [PMID: 11479279] |
4. |
Tiralongo, J., Fujita, A., Sato, C., Kitajima, K., Lehmann, F., Oschlies, M., Gerardy-Schahn, R. and Munster-Kuhnel, A.K. The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme. Glycobiology 17 (2007) 945–954. [DOI] [PMID: 17580313] |
5. |
Wang, L., Lu, Z., Allen, K.N., Mariano, P.S. and Dunaway-Mariano, D. Human symbiont Bacteroides thetaiotaomicron synthesizes 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Chem. Biol. 15 (2008) 893–897. [DOI] [PMID: 18804026] |
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[EC 2.7.7.92 created 2016] |
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EC |
3.1.3.103 |
Accepted name: |
3-deoxy-D-glycero-D-galacto-nonulopyranosonate 9-phosphatase |
Reaction: |
3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate 9-phosphate + H2O = 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate + phosphate |
Other name(s): |
3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate-9-phosphate phosphatase |
Systematic name: |
3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate 9-phosphohydrolase |
Comments: |
The enzyme, characterized from the bacterium Bacteroides thetaiotaomicron, is part of the biosynthesis pathway of the sialic acid 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate (Kdn). Kdn is abundant in extracellular glycoconjugates of lower vertebrates such as fish and amphibians, but is also found in the capsular polysaccharides of bacteria that belong to the Bacteroides genus. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Wang, L., Lu, Z., Allen, K.N., Mariano, P.S. and Dunaway-Mariano, D. Human symbiont Bacteroides thetaiotaomicron synthesizes 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Chem. Biol. 15 (2008) 893–897. [DOI] [PMID: 18804026] |
2. |
Lu, Z., Wang, L., Dunaway-Mariano, D. and Allen, K.N. Structure-function analysis of 2-keto-3-deoxy-D-glycero-D-galactonononate-9-phosphate phosphatase defines specificity elements in type C0 haloalkanoate dehalogenase family members. J. Biol. Chem. 284 (2009) 1224–1233. [DOI] [PMID: 18986982] |
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[EC 3.1.3.103 created 2016] |
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