EC |
1.13.11.4 |
Accepted name: |
gentisate 1,2-dioxygenase |
Reaction: |
2,5-dihydroxybenzoate + O2 = maleylpyruvate |
Other name(s): |
gentisate oxygenase; 2,5-dihydroxybenzoate dioxygenase; gentisate dioxygenase; gentisic acid oxidase; gentisate:oxygen 1,2-oxidoreductase (decyclizing) |
Systematic name: |
gentisate:oxygen 1,2-oxidoreductase (ring-opening) |
Comments: |
Requires Fe2+. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9029-48-5 |
References: |
1. |
Hayaishi, O. Direct oxygenation by O2, oxygenases. In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Ed.), The Enzymes, 2nd edn, vol. 8, Academic Press, New York, 1963, pp. 353–371. |
2. |
Sugiyama, S., Yano, K., Komagata, K. and Arima, K. Metabolites of aromatic compounds by microbes. Part VII. Gentisic acid oxidase. Bull. Agric. Chem. Soc. Jpn 24 (1960) 243–248. |
3. |
Sugiyama, S., Yano, K. and Arima, K. Metabolites of aromatic compounds by microbes. Part VII. Further studies of gentisic acid oxidase. Bull. Agric. Chem. Soc. Jpn 24 (1960) 249–254. |
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[EC 1.13.11.4 created 1961 as EC 1.99.2.4, transferred 1965 to EC 1.13.1.4, transferred 1972 to EC 1.13.11.4] |
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EC |
1.14.13.24 |
Accepted name: |
3-hydroxybenzoate 6-monooxygenase |
Reaction: |
3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O |
Other name(s): |
3-hydroxybenzoate 6-hydroxylase; m-hydroxybenzoate 6-hydroxylase; 3-hydroxybenzoic acid-6-hydroxylase |
Systematic name: |
3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating) |
Comments: |
A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly. |
Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 51570-26-4 |
References: |
1. |
Groseclose, E.E. and Ribbons, D.W. 3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 55 (1973) 897–903. [DOI] [PMID: 4357436] |
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[EC 1.14.13.24 created 1984] |
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EC |
1.14.13.172 |
Accepted name: |
salicylate 5-hydroxylase |
Reaction: |
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O |
Glossary: |
2,5-dihydroxybenzoate = gentisate |
Other name(s): |
nagG (gene name); nagH (gene name) |
Systematic name: |
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating) |
Comments: |
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin—NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin. |
Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Fuenmayor, S.L., Wild, M., Boyes, A.L. and Williams, P.A. A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2. J. Bacteriol. 180 (1998) 2522–2530. [PMID: 9573207] |
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[EC 1.14.13.172 created 2013] |
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EC |
1.14.13.209 |
Accepted name: |
salicyloyl-CoA 5-hydroxylase |
Reaction: |
2-hydroxybenzoyl-CoA + NADH + H+ + O2 = gentisyl-CoA + NAD+ + H2O |
Glossary: |
2-hydroxybenzoyl-CoA = salicyloyl-CoA
gentisate = 2,5-dihydroxybenzoate |
Other name(s): |
sdgC (gene name) |
Systematic name: |
salicyloyl-CoA,NADH:oxygen oxidoreductase (5-hydroxylating) |
Comments: |
The enzyme, characterized from the bacterium Streptomyces sp. WA46, participates in a pathway for salicylate degradation. cf. EC 1.14.13.172, salicylate 5-hydroxylase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Ishiyama, D., Vujaklija, D. and Davies, J. Novel pathway of salicylate degradation by Streptomyces sp. strain WA46. Appl. Environ. Microbiol. 70 (2004) 1297–1306. [DOI] [PMID: 15006746] |
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[EC 1.14.13.209 created 2015] |
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EC |
4.1.1.62 |
Accepted name: |
gentisate decarboxylase |
Reaction: |
2,5-dihydroxybenzoate = hydroquinone + CO2 |
Glossary: |
gentisate = 2,5-dihydroxybenzoate |
Other name(s): |
2,5-dihydroxybenzoate decarboxylase; gentisate carboxy-lyase |
Systematic name: |
2,5-dihydroxybenzoate carboxy-lyase (hydroquinone-forming) |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 37290-54-3 |
References: |
1. |
Grant, D.J.W. and Patel, J.C. Non-oxidative decarboxylation of p-hydroxybenzoic acid, gentisic acid, protocatechuic acid, and gallic acid by Klebsiella aerogenes (Aerobacter aerogenes). J. Microbiol. Serol. 35 (1969) 325–343. [PMID: 5309907] |
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[EC 4.1.1.62 created 1972] |
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