EC |
6.4.1.7 |
Accepted name: |
2-oxoglutarate carboxylase |
Reaction: |
ATP + 2-oxoglutarate + HCO3- = ADP + phosphate + oxalosuccinate |
|
For diagram of reaction, click here |
Glossary: |
oxalosuccinate = 1-oxopropane-1,2,3-tricarboxylate |
Other name(s): |
oxalosuccinate synthetase; carboxylating factor for ICDH (incorrect); CFI; OGC |
Comments: |
A biotin-containing enzyme that requires Mg2+ for activity. It was originally thought [1] that this enzyme was a promoting factor for the carboxylation of 2-oxoglutarate by EC 1.1.1.41, isocitrate dehydrogenase (NAD+), but this has since been disproved [2]. The product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 [2]. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 60382-75-4 |
References: |
1. |
Aoshima, M., Ishii, M. and Igarashi, Y. A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6. Mol. Microbiol. 51 (2004) 791–798. [DOI] [PMID: 14731279] |
2. |
Aoshima, M. and Igarashi, Y. A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6. Mol. Microbiol. 62 (2006) 748–759. [DOI] [PMID: 17076668] |
|
[EC 6.4.1.7 created 2006] |
|
|
|
|