ExplorEnz: EC 6.3.1.8

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6.3.1.8

Accepted name:

glutathionylspermidine synthase

Reaction:

glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate

For diagram of trypanothione biosynthesis, click here and for diagram of trypanothione biosynthesis, click here

Glossary:

glutathione = γ-L-glutamyl-L-cysteinyl-glycine
spermidine = N-(3-aminopropyl)butane-1,4-diamine

Other name(s):

glutathione:spermidine ligase (ADP-forming)

Systematic name:

γ-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP-forming) [spermidine is numbered so that atom N-1 is in the amino group of the aminopropyl part of the molecule]

Comments:

Requires magnesium ions. Involved in the synthesis of trypanothione in trypanosomatids. The enzyme from Escherichia coli is bifunctional and also catalyses the glutathionylspermidine amidase (EC 3.5.1.78) reaction, resulting in a net hydrolysis of ATP.

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 9077-09-2

References:

1.	Smith, K., Nadeau, K., Bradley, M., Walsh, C.T., Fairlamb, A.H. Purification of glutathionylspermidine and trypanothione synthase from Crithidia fasciculata. Protein Sci. 1 (1992) 874–883. [DOI] [PMID: 1304372]
2.	Bollinger, J.M., Kwon, D.S., Huisman, G.W., Kolter, R., Walsh, C.T. Glutathionylspermidine metabolism in E. coli. Purification, cloning, overproduction and characterization of a bifunctional glutathionylspermidine synthetase/amidase. J. Biol. Chem. 270 (1995) 14031–14041. [DOI] [PMID: 7775463]

[EC 6.3.1.8 created 1999]