The Enzyme Database

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EC 6.3.1.22     
Accepted name: tRNAmet cytidine acetate ligase
Reaction: ATP + [elongator tRNAMet]-cytidine34 + acetate = AMP + diphosphate + [elongator tRNAMet]-N4-acetylcytidine34 (overall reaction)
(1a) ATP + acetate = acetyladenylate + diphosphate
(1b) acetyladenylate + [elongator tRNAMet]-cytidine34 = AMP + [elongator tRNAMet]-N4-acetylcytidine34
Other name(s): tmcAL (gene name)
Systematic name: elongator tRNAmet cytidine:acetate ligase (AMP-forming)
Comments: The enzyme, charactrized from the bacterium Bacillus subtilis, catalyses a similar tRNA modification to that performed by EC 2.3.1.193, tRNAMet cytidine acetyltransferase. However, unlike that enzyme, which uses acetyl-CoA as the acetyl donor, this enzyme activates an acetate ion to form acetyladenylate and then catalyses the acetylation through a mechanism similar to tRNA aminoacylation.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
References:
1.  Taniguchi, T., Miyauchi, K., Sakaguchi, Y., Yamashita, S., Soma, A., Tomita, K. and Suzuki, T. Acetate-dependent tRNA acetylation required for decoding fidelity in protein synthesis. Nat. Chem. Biol. 14 (2018) 1010–1020. [DOI] [PMID: 30150682]
[EC 6.3.1.22 created 2024]
 
 


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