| EC |
6.3.1.15 |
| Accepted name: |
8-demethylnovobiocic acid synthase |
| Reaction: |
ATP + 4-hydroxy-3-prenylbenzoate + 3-amino-4,7-dihydroxycoumarin = AMP + diphosphate + 8-demethylnovobiocic acid |
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For diagram of novobiocin biosynthesis, click here |
| Glossary: |
8-demethylnovobiocic acid = N-(2,7-dihydroxy-4-oxochromen-3-yl)-4-hydroxy-3-(3-methylbut-2-en-1-yl)benzamide |
| Other name(s): |
novL (gene name); novobiocin ligase; novobiocic acid synthetase (misleading); 8-desmethyl-novobiocic acid synthetase; 8-demethylnovobiocic acid synthetase; 3-dimethylallyl-4-hydroxybenzoate:3-amino-4,7-dihydroxycoumarin ligase (AMP-forming) |
| Systematic name: |
4-hydroxy-3-prenylbenzoate:3-amino-4,7-dihydroxycoumarin ligase (AMP-forming) |
| Comments: |
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Steffensky, M., Li, S.M. and Heide, L. Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891. J. Biol. Chem. 275 (2000) 21754–21760. [DOI] [PMID: 10801869] |
| 2. |
Pi, N., Meyers, C.L., Pacholec, M., Walsh, C.T. and Leary, J.A. Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton. Proc. Natl. Acad. Sci. USA 101 (2004) 10036–10041. [DOI] [PMID: 15218104] |
| 3. |
Pacholec, M., Tao, J. and Walsh, C.T. CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis. Biochemistry 44 (2005) 14969–14976. [DOI] [PMID: 16274243] |
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| [EC 6.3.1.15 created 2013] |
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