| EC |
6.2.1.42 |
| Accepted name: |
3-oxocholest-4-en-26-oate—CoA ligase |
| Reaction: |
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA |
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For diagram of cholic acid biosynthesis (sidechain), click here |
| Other name(s): |
fadD19 (gene name) |
| Systematic name: |
(25S)-3-oxocholest-4-en-26-oate:CoA ligase (AMP-forming) |
| Comments: |
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3β-hydroxycholest-5-en-26-oate can also be used as substrate. |
| Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Wilbrink, M.H., Petrusma, M., Dijkhuizen, L. and van der Geize, R. FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains. Appl. Environ. Microbiol. 77 (2011) 4455–4464. [DOI] [PMID: 21602385] |
| 2. |
Casabon, I., Swain, K., Crowe, A.M., Eltis, L.D. and Mohn, W.W. Actinobacterial acyl coenzyme a synthetases involved in steroid side-chain catabolism. J. Bacteriol. 196 (2014) 579–587. [DOI] [PMID: 24244004] |
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| [EC 6.2.1.42 created 2014] |
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