|
|
Your query returned 1 entry. 
Printable version
| EC | 6.1.2.2 | ||
| Accepted name: | nebramycin 5′ synthase | ||
| Reaction: | (1) tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5′ + AMP + diphosphate + phosphate (overall reaction) (1a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate (1b) O-carbamoyladenylate + tobramycin = AMP + nebramycin 5′ (2) kanamycin A + carbamoyl phosphate + ATP + H2O = 6′′-O-carbamoylkanamycin A + AMP + diphosphate + phosphate (overall reaction) (2a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate (2b) O-carbamoyladenylate + kanamycin A = AMP + 6′′-O-carbamoylkanamycin A |
||
| For diagram of kanamycin A biosynthesis, click here | |||
| Glossary: | tobramycin = (1S,2S,3R,4S,6R)-4,6-diamino-3-(2,6-diamino-2,3,6-trideoxy-α-D-ribo-hexopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-α-D-glucopyranoside nebramycin 5′ = (1S,2S,3R,4S,6R)-4,6-diamino-3-[(2,6-diamino-2,3,6-trideoxy-α-D-ribo-hexopyranosyl)oxy]-2-hydroxycyclohexyl 3-amino-6-O-carbamoyl-3-deoxy-α-D-glucopyranoside kanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy--D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy--D-glucopyranoside 6′′-O-carbamoylkanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-[(6-amino-6-deoxy-α-D-glucopyranosyl)oxy]-2-hydroxycyclohexyl 3-amino-6-O-carbamoyl-3-deoxy-α-D-glucopyranoside |
||
| Other name(s): | tobramycin carbamoyltransferase; TobZ | ||
| Systematic name: | tobramycin:carbamoyl phosphate ligase (AMP,phosphate-forming) | ||
| Comments: | Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin. | ||
| Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||
| References: |
| ||