ExplorEnz: EC 5.4.99.27

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5.4.99.27

Accepted name:

tRNA pseudouridine¹³ synthase

Reaction:

tRNA uridine¹³ = tRNA pseudouridine¹³

Other name(s):

TruD; YgbO; tRNA PSI13 synthase; RNA:PSI-synthase Pus7p; Pus7p; RNA:pseudouridine-synthase Pus7p; Pus7 protein

Systematic name:

tRNA-uridine¹³ uracil mutase

Comments:

Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine¹³ in tRNA [2,3]. The Pus7 protein from Saccharomyces cerevisiae is a multisite-multisubstrate pseudouridine synthase that is able to modify uridine¹³ in several yeast tRNAs, uridine³⁵ in the pre-tRNA^Tyr, uridine³⁵ in U2 small nuclear RNA, and uridine⁵⁰ in 5S rRNA [5].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 430429-15-5

References:

1.	Ericsson, U.B., Nordlund, P. and Hallberg, B.M. X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold. FEBS Lett. 565 (2004) 59–64. [DOI] [PMID: 15135053]
2.	Chan, C.M. and Huang, R.H. Enzymatic characterization and mutational studies of TruD—the fifth family of pseudouridine synthases. Arch. Biochem. Biophys. 489 (2009) 15–19. [DOI] [PMID: 19664587]
3.	Kaya, Y. and Ofengand, J. A novel unanticipated type of pseudouridine synthase with homologs in bacteria, archaea, and eukarya. RNA 9 (2003) 711–721. [DOI] [PMID: 12756329]
4.	Behm-Ansmant, I., Urban, A., Ma, X., Yu, Y.T., Motorin, Y. and Branlant, C. The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Ψ-synthase also acting on tRNAs. RNA 9 (2003) 1371–1382. [DOI] [PMID: 14561887]
5.	Urban, A., Behm-Ansmant, I., Branlant, C. and Motorin, Y. RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:Ψ-synthase Pus7p. J. Biol. Chem. 284 (2009) 5845–5858. [DOI] [PMID: 19114708]

[EC 5.4.99.27 created 2011]