| EC |
4.6.1.16 |
| Accepted name: |
tRNA-intron lyase |
| Reaction: |
pretRNA = a 3′-half-tRNA molecule with a 5′-OH end + a 5′-half-tRNA molecule with a 2′,3′-cyclic phosphate end + an intron with a 2′,3′-cyclic phosphate and a 5′-hydroxyl terminus |
| Other name(s): |
transfer ribonucleate intron endoribonuclease; tRNA splicing endonuclease; splicing endonuclease; tRNATRPintron endonuclease; transfer splicing endonuclease |
| Systematic name: |
pretRNA lyase (intron-removing; cyclic-2′,3′-phosphate-forming) |
| Comments: |
The enzyme catalyses the final stage in the maturation of tRNA molecules. |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 117444-13-0 |
| References: |
| 1. |
Attardi, D.G., Margarit, I. and Tocchini-Valentini, G.P. Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease. EMBO J. 4 (1985) 3289–3297. [PMID: 3937725] |
| 2. |
Peebles, C.L., Gegenheimer, P. and Abelson, J. Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease. Cell 32 (1983) 525–536. [DOI] [PMID: 6186398] |
| 3. |
Thompson, L.D., Brandon, L.D., Nieuwlandt, D.T. and Daniels, C.J. Transfer RNA intron processing in the halophilic archaebacteria. Can. J. Microbiol. 35 (1989) 36–42. [PMID: 2470486] |
| 4. |
Thompson, L.D. and Daniels, C.J. A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties. J. Biol. Chem. 263 (1988) 17951–17959. [PMID: 3192521] |
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| [EC 4.6.1.16 created 1992 as EC 3.1.27.9, transferred 2014 to EC 4.6.1.16] |
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