ExplorEnz: EC 4.2.3.42

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4.2.3.42

Accepted name:

aphidicolan-16β-ol synthase

Reaction:

9α-copalyl diphosphate + H₂O = aphidicolan-16β-ol + diphosphate

For diagram of diterpenoid biosynthesis, click here and for reaction mechanism, click here

Other name(s):

PbACS

Systematic name:

9α-copalyl-diphosphate diphosphate-lyase (aphidicolan-16β-ol-forming)

Comments:

This is a bifunctional enzyme which also has EC 5.5.1.14 syn-copalyl diphosphate synthase activity. Aphidicolan-16β-ol is a precursor of aphidicolin, a specific inhibitor of DNA polymerase α (EC 2.7.7.7).

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Oikawa, H., Toyomasu, T., Toshima, H., Ohashi, S., Kawaide, H., Kamiya, Y., Ohtsuka, M., Shinoda, S., Mitsuhashi, W. and Sassa, T. Cloning and functional expression of cDNA encoding aphidicolan-16 β-ol synthase: a key enzyme responsible for formation of an unusual diterpene skeleton in biosynthesis of aphidicolin. J. Am. Chem. Soc. 123 (2001) 5154–5155. [PMID: 11457369]
2.	Toyomasu, T., Nakaminami, K., Toshima, H., Mie, T., Watanabe, K., Ito, H., Matsui, H., Mitsuhashi, W., Sassa, T. and Oikawa, H. Cloning of a gene cluster responsible for the biosynthesis of diterpene aphidicolin, a specific inhibitor of DNA polymerase α. Biosci. Biotechnol. Biochem. 68 (2004) 146–152. [DOI] [PMID: 14745177]

[EC 4.2.3.42 created 2009]