ExplorEnz: EC 4.2.2.5

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4.2.2.5

Accepted name:

chondroitin AC lyase

Reaction:

Eliminative degradation of polysaccharides containing 1,4-β-D-hexosaminyl and 1,3-β-D-glucuronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups

Glossary:

chondroitin sulfate A = chondroitin 4-sulfate
chondroitin sulfate C = chondroitin 6-sulfate
For the nomenclature of glycoproteins, glycopeptides and peptidoglycans, click here

Other name(s):

chondroitinase (ambiguous); chondroitin sulfate lyase; chondroitin AC eliminase; chondroitinase AC; ChnAC

Systematic name:

chondroitin AC lyase

Comments:

Acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate. In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(β1-3)GalNAc(β1-]_n, which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(α1-3)GalNAc(β1-]_n of DS. Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source [4].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 9047-57-8

References:

1.	Nakada, H.I. and Wolfe, J.B. Studies on the enzyme chondroitinase: product structure and ion effects. Arch. Biochem. Biophys. 94 (1961) 244–251. [DOI] [PMID: 13727579]
2.	Pojasek, K., Shriver, Z., Kiley, P., Venkataraman, G. and Sasisekharan, R. Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum. Biochem. Biophys. Res. Commun. 286 (2001) 343–351. [DOI] [PMID: 11500043]
3.	Fethiere, J., Shilton, B.H., Li, Y., Allaire, M., Laliberte, M., Eggimann, B. and Cygler, M. Crystallization and preliminary analysis of chondroitinase AC from Flavobacterium heparinum. Acta Crystallogr. D Biol. Crystallogr. 54 (1998) 279–280. [PMID: 9761894]
4.	Huckerby, T.N., Nieduszynski, I.A., Giannopoulos, M., Weeks, S.D., Sadler, I.H. and Lauder, R.M. Characterization of oligosaccharides from the chondroitin/dermatan sulfates. ¹H-NMR and ¹³C-NMR studies of reduced trisaccharides and hexasaccharides. FEBS J. 272 (2005) 6276–6286. [DOI] [PMID: 16336265]

[EC 4.2.2.5 created 1972 (EC 4.2.99.6 created 1965, part incorporated 1976)]