The Enzyme Database

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Accepted name: difructose-anhydride-I synthase
Reaction: inulobiose = bis-D-fructose 2′,1:2,1′-dianhydride + H2O
Glossary: α-D-fructofuranose-β-D-fructofuranose 2′,1:2,1′-dianhydride = bis-D-fructose 2′,1:2,1′-dianhydride = difructose anhydride I = DFA-I
Other name(s): DFAIase; inulobiose hydrolase; bis-D-fructose 2′,1:2,1′-dianhydride fructohydrolase
Systematic name: inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2′,1:2,1′-dianhydride-forming)
Comments: The enzyme, studied in the fungus Aspergillus fumigatus, may participate in an inulin degradation pathway in which it converts the product of EC, inulin fructotransferase (DFA-I-forming), to inulobiose, though in vitro activity was higher in the direction of DFA-I formation.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 121479-55-8
1.  Matsuyama, T. and Tanaka, K. On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose. Agric. Biol. Chem. 53 (1989) 831–832.
2.  Matsuyama, T., Tanaka, K., Mashiko, M. and Kanamoto, M. Enzymic formation of di-D-fructose 1,2′; 2,1′ dianhydride from inulobiose by Aspergillus fumigatus. J. Biochem. (Tokyo) 92 (1982) 1325–1328. [PMID: 6757245]
[EC created 1992 as EC, transferred to EC 2021]

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