The Enzyme Database

Your query returned 1 entry.    printer_iconPrintable version

EC 4.2.1.157     
Accepted name: (R)-2-hydroxyisocaproyl-CoA dehydratase
Reaction: (R)-2-hydroxy-4-methylpentanoyl-CoA = 4-methylpent-2-enoyl-CoA + H2O
Other name(s): 2-hydroxyisocaproyl-CoA dehydratase; HadBC
Systematic name: (R)-2-hydroxy-4-methylpentanoyl-CoA hydro-lyase
Comments: The enzyme, isolated from the bacterium Peptoclostridium difficile, is involved in the reductive branch of L-leucine fermentation. It catalyses an α/β-dehydration, which depends on the reductive formation of ketyl radicals on the substrate generated by injection of a single electron from the ATP-dependent activator protein HadI.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
References:
1.  Kim, J., Darley, D. and Buckel, W. 2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile. FEBS J. 272 (2005) 550–561. [DOI] [PMID: 15654892]
2.  Knauer, S.H., Buckel, W. and Dobbek, H. Structural basis for reductive radical formation and electron recycling in (R)-2-hydroxyisocaproyl-CoA dehydratase. J. Am. Chem. Soc. 133 (2011) 4342–4347. [DOI] [PMID: 21366233]
[EC 4.2.1.157 created 2015]
 
 


Data © 2001–2024 IUBMB
Web site © 2005–2024 Andrew McDonald