ExplorEnz: EC 4.2.1.140

Your query returned 1 entry. Printable version

4.2.1.140

Accepted name:

gluconate/galactonate dehydratase

Reaction:

(1) D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H₂O
(2) D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H₂O

For diagram of the Entner-Doudoroff pathway, click here

Other name(s):

gluconate dehydratase (ambiguous); Sso3198 (gene name); Pto0485 (gene name)

Systematic name:

D-gluconate/D-galactonate hydro-lyase

Comments:

The enzyme is involved in glucose and galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway in Picrophilus torridus [3] and via the branched variant of the Entner-Doudoroff pathway in Sulfolobus solfataricus [1,2]. In vitro it utilizes D-gluconate with 6-10 fold higher catalytic efficiency than D-galactonate [1,3]. It requires Mg²⁺ for activity [1,2]. cf. EC 4.2.1.6, galactonate dehydratase, and EC 4.2.1.39, gluconate dehydratase.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Lamble, H.J., Milburn, C.C., Taylor, G.L., Hough, D.W. and Danson, M.J. Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus. FEBS Lett. 576 (2004) 133–136. [DOI] [PMID: 15474024]
2.	Ahmed, H., Ettema, T.J., Tjaden, B., Geerling, A.C., van der Oost, J. and Siebers, B. The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation. Biochem. J. 390 (2005) 529–540. [DOI] [PMID: 15869466]
3.	Reher, M., Fuhrer, T., Bott, M. and Schonheit, P. The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase. J. Bacteriol. 192 (2010) 964–974. [DOI] [PMID: 20023024]

[EC 4.2.1.140 created 2013]